Following to this invention, it produces the HCV replicon duplication cell RNA which includes HCV replicon array and selected marker gene array, making use of the production manner which includes the process which it introduces into the healing cell which is derived from the Li23 cell or the Li23 cell. Furthermore, the total length HCV RNA duplication cell is produced making use of the production manner which includes the process which is introduced to the cell which is shown RNA which includes total length HCV genome array and selected marker gene array, by the healing cell which is derived from the Li23 cell or the Li23 cell. Because of this, it is possible to construct the HCV life cycle reappearance system which possesses the capacity which is a match to the HCV life cycle reappearance system of HuH-7 cell stocks origin, is derived from the cell stocks other than HuH-7 cell stocks.本発明に従って、HCVレプリコン配列および選択マーカー遺伝子配列を含んでいるRNAを、Li23細胞またはLi23細胞に由来する治癒細胞に導入する工程を包含する作製方法を用いて、HCVレプリコン複製細胞を作製する。さらに、全長HCVゲノム配列および選択マーカー遺伝子配列を含んでいるRNAを、Li23細胞またはLi23細胞に由来する治癒細胞によって示される細胞に導入する工程を包含する作製方法を用いて、全長HCV RNA複製細胞を作製する。これにより、HuH-7細胞株由来のHCV生活環再現システムに匹敵する能力を有する、HuH-7細胞株以外の細胞株に由来するHCV生活環再現システムを構築することができる。
