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Hidrogeles de PEG disolubles sensibles a estímulos biofuncionalizados
专利权人:
UNIVERSITAETSKLINIKUM FREIBURG
发明人:
TOMAKIDI, PASCAL,STEINBERG, TORSTEN,WEBER, WILFRIED,LAIRD, DOUGAL,GUEBELI, RAPHAEL
申请号:
ES10014788
公开号:
ES2423798T3
申请日:
2010.11.19
申请国别(地区):
ES
年份:
2013
代理人:
摘要:
PEG hydrogel sensitive to stimuli comprising a matrix of PEG polymers which estánmodificados to contain at least one protein multifunctional fusion protein multifunctional fusion quecomprende as components a peptide substrate binding (SBP), a peptide binding dissolvable RGD repetitive, preferably a marker for purification and at least one N and / or C-terminal connector wherein the at least one protein of multifunctional fusion is covalently bound to PEG polymer, wherein the peptide substrate binding (SBP) is selected from the N-terminal Bde subunit DNA gyrase Escherichia coli (GyrB) the N-terminus of the B subunit of DNA gyrase ofEscherichia coli according to SEQ ID No. 2 or an amino acid sequence showing at least 80% deidentidad with the sequence according to SEQ ID NO: 2, sequence or between FKBP proteins, FM, ToxT, DHFR, Cyp, E, PIP, TetR, ArgR, ArsR, HucR, FRB, streptavidin, avidin, neutravidin, steroid hormone receptor, a hormonaesteroide or protein heparin binding where the N- and / or C-terminal connector is selected from a thiol-containing moiety, one modificadocon thiol or amino thiol, a cysteine, one homocysteine, coupled to maleimide thiol moiety vinylsulfone, peptide sequences, peptide bonds, one HaloTag a SNAP-tag, one CLIP-tag, a link of detransglutaminasa reaction, amino acids or entities chelation NTA or polyhistidine binding to a iónmetálico multivalent, and where gel formation is performed by attaching the binding peptide substrate (SBP) with specific susustrato.Hidrogel de PEG disoluble sensible a estímulos que comprende una matriz de polímeros de PEG, que estánmodificados para contener al menos una proteína de fusión multifuncional, proteína de fusión multifuncional quecomprende como componentes un péptido de unión al sustrato (SBP), un péptido de unión a RGD repetitivo,preferentemente un marcador para la purificación y al menos un conector N- y/o C-terminal donde la al menos una proteína de fusión multifuncional está unida covalentemente al pol
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