The present invention provides a method for suppressing differentiation from pluripotent stem cells into endoderm-derived cells, particularly a method for inhibiting differentiation into endoderm-derived cells with a TGF inhibitor or SNAI1 inhibitor. [Selection] Figure 4本発明は、多能性幹細胞から内胚葉由来細胞への分化を抑制する方法、特にTGF阻害剤又はSNAI1阻害剤により内胚葉由来細胞への分化を阻害する方法を提供する。【選択図】 図4