TNF-α binding proteins, including chimeric, CDR-grafted, and humanized antibodies that bind TNF-α are provided. Binding proteins have high affinity for TNF-α and neutralize TNF-α activity. A binding protein can be a full-length antibody or a TNF-α -binding portion thereof. Methods of making and methods of using the binding proteins are also described. The TNF-α binding proteins are useful for detecting TNF-α and for inhibiting TNF-α activity, including in a human subject suffering from a disease or disorder in which TNF-α activity is detrimental.本發明提供TNF-α結合蛋白，包括結合TNF-α之嵌合抗體、CDR移植抗體及人類化抗體。結合蛋白對TNF-α具有高親和力且中和TNF-α活性。結合蛋白可為全長抗體或其TNF-α結合部分。亦描述該等結合蛋白之製備方法及使用方法。TNF-α結合蛋白適用於偵測TNF-α及抑制TNF-α活性，包括在罹患因TNF-α活性而受害之疾病或病症的人類個體中。
