The present invention provides a plant ferment, preparation method thereof and use thereof for liver health care. The plant ferment can effectively improve the antioxidant activity of liver cells to prevent the damage caused by the oxidative stress, and can simultaneously effectively improving the gene expression level of CCT7, CCT8, Pink1, and MRPS5 to enhance the anti-aging activity of liver cells. The plant burning ferment is prepared by fermenting the Dolichos lobatus Willd. extract and the Hovenia dulcis Thunb. extract to yeast, lactic acid bacteria, and acetic acid bacteria for three-stage fermentation.本發明提供一種植物發酵物及其製備方法與用於肝臟保健的用途，本發明之植物發酵物能有效提升肝臟細胞抗氧化活性，以預防受氧化壓力的傷害，同時能有效提升肝臟細胞中CCT7基因、CCT8基因、Pink1基因、及MRPS5基因之表現量，以提升肝臟細胞的抗老化活性；其中，該植物發酵物係將由一葛根萃取物及一枳椇子萃取物所組成之混合物以酵母菌、乳酸桿菌、及醋酸桿菌依序進行三段式發酵而獲得。
