Vector que comprende en posicion 5' una secuencia de nucleotidos terminal invertida (itr), en posicion central una secuencia codificante para una toxina unida operativamente a un promotor y en posicion 3' una itr, y donde dicho vector no comprende secuencias de nucleotidos virales de densovirus de junonia coenia distintas a dichas secuencias itr; metodo para producir particulas de densovirus de junonia coenia recombinantes y no replicativas; y el uso de dichas particulas para controlar lepidopteros.
UNIVERSITE DE MONTPELLIER 2 SCIENCES ET TECHNIQUES;INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA), GIST-BROCADES B.V.
发明人:
OGLIASTRO, MYLENE,PERRIN, AURELIE,BERGOIN, MAX,COUSSERANS, FRANCOIS,FOURNIER, PHILIPPE
申请号：
CL20130001208
公开号：
CL2013001208(A1)
申请日:
2013.05.02
申请国别(地区):
智利
年份:
2014
代理人:
摘要：
The present invention relates to a vector including: (i) an inverted terminal repeat (ITR) nucleotide sequence at the 5′position; (ii) a nucleotide sequence functionally bonded to a promoter at the central position, said nucleotide sequence coding for a toxin; and (iii) an inverted terminal repeat (ITR) nucleotide sequence at the 3′position, wherein said vector does not include any viral nucleotide sequences of Junonia coenia densovirus other than the ITR sequences according to (i) and (iii). The present invention also relates to a method for producing recombinant and nonreplicative particles of Junonia coenia densovirus (JcDNV) using such a vector. Finally, the present invention relates to the use of recombinant and nonreplicative particles of Junonia coenia densovirus produced according to the above-described method as a moth-control agent.
