WANG, SHU CHEN,王淑珍,王淑珍,DUH, PIN DER,杜平悳,杜平悳,CHANG, CHEN KAI,常振铠,常振鎧,CHEN, JUNG TSAI,陈荣才,陳榮才
申请号：
TW103146621
公开号：
TWI521061B
申请日:
2014.12.31
申请国别(地区):
TW
年份:
2016
代理人:
摘要：
The invention relates to a method of preparing fermentation crude extracts for inhibiting activity of angiotensin I -converting enzyme. It comprises steps of drying and grinding a material, and mixing the material with water to form a mixture adding 0.1% α-amylase for hydrolysis at 95℃ for 1 hour adding 0.1% glucoamylase for hydrolysis at 65℃ for 4 hour adding lactic acid bacteria to a medium containing the mixture for culturing for 24 hours centrifuging the medium and boiling a supernatant for 20 minutes and filtering the supernatant to acquire a fermentation crude extract with ability of inhibiting activity of angiotensin I -converting enzyme.本發明係有關於一種製備具有抑制血管收縮素轉換酶活性之發酵粗萃液之方法,係包含將一素材烘乾磨成粉末並與水混合成一混合液;加入0.1%澱粉酶(α-amylase)於95℃水解1小時;加入0.1%葡萄糖澱粉酶(glucoamylase)於65℃水解4小時;將一乳酸菌加入含有混合液之培養基內培養24小時進行發酵;離心培養基,取上清液並煮沸20分鐘;以及過濾上清液,以獲一發酵粗萃液係具有抑制血管收縮素轉換酶活性。(S1)‧‧‧步驟一(S2)‧‧‧步驟二(S3)‧‧‧步驟三(S4)‧‧‧步驟四(S5)‧‧‧步驟五(S6)‧‧‧步驟六
