Provided is a transfer membrane holder (2) for holding a transfer membrane (1) in a separation transfer device (100), wherein the transfer membrane holder (2) is provided with fasteners (20, 21) for fastening at least one end of the transfer membrane (1) in the direction of movement thereof, the fasteners (20, 21) being provided with elastic bodies (20a, 21a) for coming into contact with the transfer membrane (1) on the opposite side from an ejection unit (50a), and pressing members (20b, 21b) for pressing the transfer membrane (1) against the elastic bodies (20a, 21a).