Pearlman Paul S.,Conradie Alex Van Eck,Chen Changlin,Botes Adriana Leonora
申请号：
US201314139142
公开号：
US9920336(B2)
申请日:
2013.12.23
申请国别(地区):
美国
年份:
2018
代理人:
Simmons William J.`Jenkins Thomas H.
摘要：
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.
