The present invention relates to a purifying method of horse oil comprising: a step of selecting a part where a fat is properly accumulated from slaughtered horse meat, removing blood and meat, washing the part, and vacuum-packing the part a step of keeping vacuum-packed horse oil in a freezer a step of passing a selection process which removes the blood and the meat part of the horse oil kept in the freezer a step of vacuum-grinding and liquefying the selected horse oil at lower than or equal to 20°C a step of removing oxygen after fully filling the liquefied horse oil in a dissolver, sealing the dissolver, dissolving the liquefied horse oil at 35 to 80°C, filtering the liquefied horse oil, and gaining primarily purified horse oil a step of mixing a betacyclodextrin aqueous solution with 10% concentration in the primarily purified horse oil at a weight ratio of 1:1 a step of sealing the mixed primarily purified horse oil, and putting the primarily purified horse oil at 20 to 30°C for 12 to 24 hours a step of melting the put primarily purified horse oil by boiling in water, and gaining secondarily purified horse oil and a step of putting the secondarily purified horse oil in a container, and maturing the secondarily purified horse oil at 3 to 10°C for 24 hours. By providing the horse oil which is purified in a natural method in order to minimize damage to an active component of the horse oil and to remove a unique smell from the horse oil, the present invention enables to be used as a raw material for a cosmetic product with high functionality, high absorption, and high stability.COPYRIGHT KIPO 2015본 발명은 도축된 말고기로부터 지방 축적이 잘 되어 있는 부위를 선별한 후 핏물과 고기를 제거한 다음 세척 후 진공포장하는 단계 상기 진공·포장된 마유를 냉동보관하는 단계 상기 냉동보관된 마유의 핏물과 고기 부분을 재차 제거하는 선별과정을 거치는 단계 상기 선별된 마유를 20℃ 이하의 온도로 진공분쇄하여 액화하는 단계 상기 액화된 마유를 용해기에 가득 넣은 후 산소를 제거하여 밀봉한 다음 35~80℃의 온도로 용해 후 여과하여 1차 정제 마유를 얻는 단계 상기 1차 정제 마유에 10% 농도의 베타사이클로덱스트린 수용액을 1:1의 중량비로 혼합하는 단계 상기 혼합된 1차 정제 마유를 밀봉한 후 20~30℃의 온도에서 12~24시간 동안 정치하
