The present application provides a method for rejuvenation, characterized in that providing a cell to cultivate in a composition for a suitable period of time, wherein the composition is obtained by adding a chitosan solution to a culture surrounding. The present application further provides a composition for rejuvenating cells, comprising a mixture of a chitosan solution in a culture surrounding.本申請提供一種使細胞回春之方法,其特徵在於提供一細胞於一組合物中培養一適當期間,其中該組合物係將一幾丁聚醣溶液加入一細胞培養環境中獲得。本申請另提供一種組合物,用於使細胞回春,包含一幾丁聚醣溶液之一培養環境。