THE EUROPEAN MOLECULAR BIOLOGY LABORATORY;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
发明人:
Imre BERGER,Frédéric GARZONI,Pascal FENDER
申请号：
US16088905
公开号：
US20200325179A1
申请日:
2017.03.31
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The present invention relates to new adenoviral coat protein based delivery vehicles. They are based on a modified penton base protomers that assemble into VLPs. Exposed areas of the penton base proteins can be modified to allow the VLP to specifically bind to any target and/or to comprise any desired peptide epitope. Additional cargo, e.g. drugs, proteins, or nucleic acids, can reversibly or irreversibly attached to the VLP via engineered fibre protein fragments. The present invention relates to such engineered penton base protomers, engineered proteins comprising an adenovirus fibre protein N-terminal fragment specifically binding to an adenovirus fibre protein binding cleft of a penton base protomer, VLPs comprising the engineered penton base protomers and optionally engineered proteins comprising an adenovirus fibre protein N-terminal fragment specifically binding to an adenovirus fibre protein binding cleft of a penton base protomer, nucleic encoding the engineered proteins, the VLPs as well as methods of producing the proteins and VLPs.
