An integrated chip for isolating and capturing cells in one step, relating to the field of biotechnology. The integrated chip comprises: a cell enrichment region, a cell isolation region, and a cell capture region. An inlet of the cell enrichment region is provided at one end of the same, and a waste liquid outlet and an enrichment liquid outlet are provided at the other end of the cell enrichment region. A buffer inlet and an enrichment liquid inlet, in communication with the enrichment liquid outlet, are provided at one end of the cell isolation region, and an outlet of the cell isolation region is provided at the other end of the same. An inlet communicating with the outlet of the cell isolation region is provided at one end of the cell capture region, and an isolation liquid outlet is provided at the other end of the cell capture region. A cell sample to be separated enters the cell enrichment region so as to increase the concentration of a target cell in the cell sample. An enrichment liquid flowing out
