Cultivation method for raising triterpenoid component of Antrodia cinnamomea to cultivate for one month to be able to harvest Antrodia cinnamomea with large amount of triterpenoid without needing to use Antrodia basswood for cultivation
A cultivation method for raising triterpenoid component of Antrodia cinnamomea is to add a predetermined proportion of substances such as malt extract, glucose, peptone, agar and mushroom extract etc. into distilled water and mix uniformly. Then the mixed culture medium is placed in an autoclave for heating and sterilizing at a predetermined pressure and temperature for a suitable time. The sterilized culture medium is then poured into a culture dish to a suitable height in a sterile environment, and allowed to stand and cool the culture medium solution to form a solid culture medium. The strains of Antrodia cinnamomea are inoculated into different positions of the solid culture medium, with the positions at an appropriate spacing. Finally, cultivation at a predetermined temperature, wavelength, and under illumination of light intensity for one month can harvest Antrodia cinnamomea with large amount of triterpenoid without needing to use Antrodia basswood for cultivation.一種提高牛樟芝三萜類成分之培養方法,係先將預定比例之麥芽萃取物、葡萄糖、蛋白腖、瓊脂及菇類萃取物等物質加入蒸餾水後均勻混合,再將混合後之培養基溶液置於一高壓滅菌設備內,於預定壓力、溫度下加熱殺菌適當時間,繼而將滅菌後之培養基溶液,於無菌環境下倒入一培養皿至適當高度,並靜置、冷卻培養基溶液,以形成一固態培養基,再將牛樟芝菌種分別接菌至固態培養基之複數位置,各位置並具適當間距,最後,於預定溫度、波長、照射光度之光源照射下培養30天,不需要使用牛樟椴木培養,即可於1個月採收高三萜總量的牛樟芝。100‧‧‧提高牛樟芝三萜類成分之培養方法110‧‧‧混合培養基之組成成分120‧‧‧滅菌130‧‧‧形成固態培養基140‧‧‧菌株接種150‧‧‧光線刺激培養
