A method for preparing a feedstuff comprising butyric acid and/or butyrate, comprising: adding a microorganism into a light corn steepwater to provide a mixture, wherein the microorganism comprises a first strain and the first strain is able to metabolize saccharides and/or organic compounds in a fermentation to produce butyric acid keeping the mixture under an anaerobic atmosphere to conduct the fermentation to provide a fermentation broth and optionally condensing the fermentation broth. Optionally, the microorganism further comprises a second strain, wherein the second strain is able to fix a carbon oxide.一種含有丁酸及/或丁酸鹽之飼料原料的製備方法,其係包含:於一浸泡液(light steepwater)中添加一微生物以提供一混合物,其中該微生物係包含一第一菌株,該第一菌株係可於發酵反應中代謝醣類及╱或有機化合物以生成丁酸;將該混合物置於厭氧氛圍下以進行發酵反應,提供一發酵液;以及視需要濃縮該發酵液。視需要地,該微生物可更包含一第二菌株,該第二菌株係可固定碳氧化物。
