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[学术文献 ] GhLPF1 Associated Network Is Involved with Cotton Lint Percentage Regulation Revealed by the Integrative Analysis of Spatial Transcriptome 进入全文
ADVANCED SCIENCE
Cotton fibers, derived from the epidermis of the ovule, provide a sustainable natural fiber source for the textile industry. Traits related to fiber yield are predominantly determined by molecular regulations in the epidermis of the outer integument (OI) region of the cotton ovule. Here, we identify an R2R3 MYB transcription factor coding gene GhLPF1 within the QTL-LP-ChrA06 locus for lint percentage (LP, percentage of lint to seed cotton) through constructing the 1-Day Post Anthesis Cotton Ovule Spatial Transcriptome Atlas. GhLPF1 is subjected as a downstream target of miR828 during fiber development. The direct downstream genes (DDGs) of GhLPF1 are biased to increased expression in GhLPF1-CR, and are preferentially expressed in OI, so that GhLPF1 is primarily a transcriptional repressor to its DDGs. Population-wide transcriptome analysis confirms that expression variation of GhLPF1-DDGs is significantly biased to negative correlation with LP, among which a type I homeobox protein-coding gene GhHB6 is further validated to be the directly downstream gene of GhLPF1. Given these data, it is demonstrated that GhLPF1 mediates a regulation network in LP as a transcriptional repressor, which makes it a valuable functional marker for fiber-trait improvement application from QTL-LP-ChrA06.
[学术文献 ] Insights into the Role of GhTAT2 Genes in Tyrosine Metabolism and Drought Stress Tolerance in Cotton 进入全文
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Gossypium hirsutum is a key fiber crop that is sensitive to environmental factors, particularly drought stress, which can reduce boll size, increase flower shedding, and impair photosynthesis. The aminotransferase (AT) gene is essential for abiotic stress tolerance. A total of 3 Gossypium species were analyzed via genome-wide analysis, and the results unveiled 103 genes in G. hirsutum, 47 in G. arboreum, and 53 in G. raimondii. Phylogenetic analysis, gene structure examination, motif analysis, subcellular localization prediction, and promoter analysis revealed that the GhAT genes can be classified into five main categories and play key roles in abiotic stress tolerance. Using RNA-seq expression and KEGG enrichment analysis of GhTAT2, a coexpression network was established, followed by RT-qPCR analysis to identify hub genes. The RT-qPCR results revealed that the genes Gh_A13G1261, Gh_D13G1562, Gh_D10G1155, Gh_A10G1320, and Gh_D06G1003 were significantly upregulated in the leaf and root samples following drought stress treatment, with Gh_A13G1261 identified as the hub gene. The GhTAT2 genes were considerably enriched for tyrosine, cysteine, methionine, and phenylalanine metabolism and isoquinoline alkaloid, tyrosine, tryptophan, tropane, piperidine, and pyridine alkaloid biosynthesis. Under drought stress, KEGG enrichment analysis manifested significant upregulation of amino acids such as L-DOPA, L-alanine, L-serine, L-homoserine, L-methionine, and L-cysteine, whereas metabolites such as maleic acid, p-coumaric acid, quinic acid, vanillin, and hyoscyamine were significantly downregulated. Silencing the GhTAT2 gene significantly affected the shoot and root fresh weights of the plants compared with those of the wild-type plants under drought conditions. RT-qPCR analysis revealed that GhTAT2 expression in VIGS-treated seedlings was lower than that in both wild-type and positive control plants, indicating that silencing GhTAT2 increases sensitivity to drought stress. In summary, this thorough analysis of the gene family lays the groundwork for a detailed study of the GhTAT2 gene members, with a specific focus on their roles and contributions to drought stress tolerance.
[学术文献 ] Cotton RLP6 Interacts With NDR1/HIN6 to Enhance Verticillium Wilt Resistance via Altering ROS and SA 进入全文
MOLECULAR PLANT PATHOLOGY
Cotton Verticillium wilt (VW) is often a destructive disease that results in significant fibre yield and quality losses in Gossypium hirsutum. Transferring the resistance trait of Gossypium barbadense to G. hirsutum is optional but challenging in traditional breeding due to limited molecular dissections of resistance genes. Here, we discovered a species-diversified structural variation (SV) in the promoter of receptor-like protein 6 (RLP6) that caused distinctly higher expression level of RLP6 in G. barbadense with the SV than G. hirsutum without the SV. Functional experiments showed that RLP6 is an important regulator in mediating VW resistance. Overexpressing RLP6 significantly enhanced resistance and root growth, whereas the opposite phenotype appeared in RLP6-silenced cotton. A series of experiments indicated that RLP6 regulated reactive oxygen species (ROS) and salicylic acid (SA) signalling, which induced diversified defence-related gene expression with pathogenesis-related (PR) proteins and cell wall proteins enrichments for resistance improvement. These findings could be valuable for the transfer of the G. barbadense SV locus to improve G. hirsutum VW resistance in future crop disease resistance breeding.
[学术文献 ] COBRA-LIKE 9 modulates cotton cell wall development via regulating cellulose deposition 进入全文
PLANT PHYSIOLOGY
Plant cell walls are complex and dynamic cellular structures critical for plant growth, development, physiology, and adaptation. Cellulose is one of the most important components of the cell wall. However, how cellulose microfibrils deposit and assemble into crystalline cellulose remains elusive. The COBRA-LIKE plant-specific protein family plays a vital role in modulating the deposition and orientation of cellulose microfibril in plant cell walls. Here, we investigate the role of GhCOBL9 in cotton (Gossypium hirsutum) fiber development, an ideal model for studying cell elongation and cell wall thickening. The expression period of GhCOBL9 is consistent with the thickening stage of the secondary wall of cotton fibers. Overexpression of GhCOBL9 results in increased cellulose content in the cell wall and produces shorter, thicker, and stronger fibers, while RNA interference (RNAi)-mediated downregulation of GhCOBL9 leads to the opposite phenotypes, indicating its crucial role in cell wall development. Subcellular localization and binding activity assays reveal that GhCOBL9 targets the cell wall and binds to crystalline cellulose with high affinity. Transcriptomic analysis of GhCOBL9 transgenic lines uncovers expression alterations in genes related to cellulose and monosaccharide biosynthesis. Furthermore, we identify a fasciclin-like arabinogalactan protein 9 (GhFLA9) as an interacting partner of GhCOBL9 to modulate cell wall development. Additionally, the R2R3-MYB transcription factor GhMYB46-5 activates GhCOBL9 expression by binding to the MYB46-responsive cis-regulatory element in the GhCOBL9 promoter. These findings broaden our knowledge of COBL function in modulating plant cell wall development. COBRA-LIKE protein interacts with a fasciclin-like arabinogalactan protein to modulate cellulose deposition and regulate fiber cell wall development in cotton.
[学术文献 ] The impact of temperature on cotton yield and production in Xinjiang, China 进入全文
npj Sustainable Agriculture
Cotton production in Xinjiang is crucial to China’s economy, but the region’s cold climate poses challenges to cultivation. This study analyzes temperature data from 33 meteorological stations in Xinjiang (1981–2020) alongside cotton yield data to assess cold damage during key cotton growth stages. A comparison is made with cotton-producing counties in the U.S. southwest (Texas, Kansas, Oklahoma). Results show that Xinjiang has a shorter frost-free period (140–210 days) compared to the U.S. (235–300 days). The Pearson correlation coefficient indicates that spring cold damage (SpCD) during emergence stage significantly impacts yield. SpCD lasts 5–10 days in NXJ, 3–7 days in SXJ, and 3–4 days in the U.S. Severe cold damage, notably in 1996 and 2010, led to a 40% yield decline. To mitigate cold damage, breeding cold-tolerant cotton varieties and developing innovative cultivation technologies are critical for sustaining cotton production in Xinjiang.
[学术文献 ] Combining suitable brown lint color, fiber quality, and yield in F2 cotton hybrids 进入全文
INDUSTRIAL CROPS AND PRODUCTS
Naturally colored cotton is eco-friendly because its use in textiles does not require dyeing, and textiles made from colored cotton are the best for human health. Despite their many advantages, the low yields and unfavorable quality traits of colored cotton varieties limit extensive production. Therefore, F1 and F2 populations of inter- specific ( Gossypium hirsutum L. x Gossypium barbadense L.) and intraspecific ( Gossypium hirsutum L.) cotton hybrids were examined to develop brown-colored cotton varieties with superior traits. Gelincik and Nazilli Deve T & uuml;y & uuml; (NDT)-15 varieties ( Gossypium hirsutum L.) with brown-colored lint were used as female parents. The male parents included Giza-75, Bahar-82 ( Gossypium barbadense L.), Fiona, May-455, I(center dot)pek-607 and Claudia ( Gossypium hirsutum L.) varieties with white-colored lint. According to the line x tester mating design, 12 hybrid combinations were obtained. F1 populations had higher yield and fiber length values than their parents and F2 populations. F2 populations had more unfavorable color values (L: whiteness, a*: red-green, and b*: yellow-blue) than their colored parents but were superior to F1s. The ratio of general combination ability variance to specific combination ability variance was less than 1, indicating that yield, ginning out-turn, fiber quality, and color parameters were controlled by non-additive genes. In the biplot graph, color and fiber parameters are located in the opposite direction from yield, indicating that it is difficult to develop colored cotton with high yield and fiber quality. Among the 960 F2 single plants evaluated, ten plants were carefully selected according to the optimization of yield, fiber quality, and color values. Based on potential diallel crosses of the ten selected plants, it was decided to begin a recurrent selection with that population. Furthermore, it was determined that F2 seeds of Nazilli DT-15 x I(center dot)pek-607 and Gelincik x Giza-75 combinations may be successfully given to farmers.
