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[学术文献 ] Evaluating Total Phenolic Content, Antioxidant Activity, High Molecular Weight Glutenin Subunits (HMW-GS), and Grain Yield Parameters of Cultivated Wheat and Hybrids 进入全文
SpringerLink
This study was conducted to assess the high molecular weight glutenin subunits (HMW-GS), yield, total phenolic content (TPC), and antioxidant capacity using five assays: free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), and phosphomolybdenum. Wheat species, old cultivar, landrace, and hybrid wheat genotypes were compared with adaptive modern bread and durum wheat cultivars. Under rainfed conditions, high grain yield was particularly obtained from T. turanicum, T. polonicum, and T. compactum wheat species, as T. spelta, T. compactum, T. turanicum, T. turgidum, and T. polonicum were better performed to grain yield under irrigated conditions. In the study, the wheat genotypes, T. petropavlovskyi, T. spelta, T. sphaerococcum, T. compactum, Yektay 406, Ak 702, Köse 220/39, and wheat hybrids were found to have the HMW-GS 2 + 12 in Glu-D1 in relation to low quality. The Yellowstone wheat cultivar carried high-quality alleles together for 1 in Glu-A1 and 5 + 10 in Glu-D1. The study exhibited that T. monococcum (einkorn), T. spelta (spelt), T. dicoccum (emmer), and Yektay 406 (old cultivar) for better antioxidant capacity were displayed together in the same cluster of the dendrogram constructed by DPPH, ABTS, CUPRAC, FRAP, and phosphomolybdenum assay results. In addition, significant correlations were observed between TPC, ABTS, CUPRAC, FRAP, and phosphomolybdenum. The study suggested that ancient wheat species superior to the investigated characteristics had antioxidants beneficial for healthy nutrition and may also be used in the improvement of cultivars with high yield and quality.
[学术文献 ] The conformation of glutenin polymers in wheat grain: some genetic and environmental factors associated with this important characteristic 进入全文
OXFORD ACADEMlC
In a previous study we used asymmetric-flow field-flow fractionation to determine the polymer mass (Mw), gyration radius (Rw) and the polydispersity index of glutenin polymers (GPs) in wheat (Triticum aestivum). Here, using the same multi-location trials (4 years, 11 locations, and 192 cultivars), we report the factors that are associated with the conformation (Conf) of the polymers, which is the slope of Log(Rw) versus a function of Log(Mw). We found that Conf varied between 0.285 and 0.740, it had low broad-sense heritability (H2=16.8), and it was significantly influenced by the temperature occurring over the last month of grain filling. Higher temperatures were found to increase Rw and the compactness and sphericity of GPs. Alleles for both high- and low-molecular-weight glutenin subunits had a significant influence on the Conf value. Assuming a Gaussian distribution for Mw, the number of polymers present in wheat grains was computed for different kernel weights and protein concentrations, and it was found to exceed 1012 GPs per grain. Using atomic force microscopy and cryo-TEM, images of GPs were obtained for the first time. Under higher average temperature, GPs became larger and more spherical and consequently less prone to rapid hydrolysis. We propose some orientations that could be aimed at potentially reducing the impact of numerous GPs on people suffering from non-celiac gluten sensitivity.
[学术文献 ] Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat 进入全文
DOAJ
As one of the most important cereals, wheat (Triticum aestivum) has high nutritional value and is widely cultivated in China. However, wheat can cause severe allergic reactions, and a growing number of people are developing allergies to Chinese wheat. Low molecular weight glutenin (LMW-GS), an important allergen in susceptible populations, is responsible for celiac disease and wheat contacts dermatitis. In this study, LMW-GS was highly purified from Chinese wheat (Xiaoyan 6) and further identified and characterized. In addition, 8 peptides were predicted efficiently by 5 immunological tools, among which five peptides showed significant immunoglobulin E (IgE) binding abilities. Two specific epitopes were found to be in the non-conserved region of the amino acid sequence of LMW-GS, which was speculated to be the specific epitope of Chinese wheat. This systematic research of LMW-GS may provide new insights into the prevention of wheat allergy and development of hypoallergenic wheat products.
[前沿资讯 ] Dual RNA-sequencing of Fusarium head blight resistance in winter wheat 进入全文
Frontiers
Fusarium head blight (FHB) is a devastating fungal disease responsible for significant yield losses in wheat and other cereal crops across the globe. FHB infection of wheat spikes results in grain contamination with mycotoxins, reducing both grain quality and yield. Breeding strategies have resulted in the production of FHB-resistant cultivars, however, the underlying molecular mechanisms of resistance in the majority of these cultivars are still poorly understood. To improve our understanding of FHB-resistance, we performed a transcriptomic analysis of FHB-resistant AC Emerson, FHB-moderately resistant AC Morley, and FHB-susceptible CDC Falcon in response to Fusarium graminearum. Wheat spikelets located directly below the point of inoculation were collected at 7-days post inoculation (dpi), where dual RNA-sequencing was performed to explore differential expression patterns between wheat cultivars in addition to the challenging pathogen. Differential expression analysis revealed distinct defense responses within FHB-resistant cultivars including the enrichment of physical defense through the lignin biosynthesis pathway, and DON detoxification through the activity of UDP-glycosyltransferases. Nucleotide sequence variants were also identified broadly between these cultivars with several variants being identified within differentially expressed putative defense genes. Further, F. graminearum demonstrated differential expression of mycotoxin biosynthesis pathways during infection, leading to the identification of putative pathogenicity factors.
[前沿资讯 ] Critical assessment of wheat biofortification for iron and zinc: a comprehensive review of conceptualization, trends, approaches, bioavailability, health impact, and policy framework 进入全文
Frontiers
Addressing global hidden hunger, particularly in women of childbearing age and children under five, presents a significant challenge, with a focus on iron (Fe) and zinc (Zn) deficiency. Wheat, a staple crop in the developing world, is crucial for addressing this issue through biofortification efforts. While extensive research has explored various approaches to enhance Fe and Zn content in wheat, there remains a scarcity of comprehensive data on their bioavailability and impact on human and animal health. This systematic review examines the latest trends in wheat biofortification approaches, assesses bioavailability, evaluates the effects of biofortified wheat on health outcomes in humans and animals, and analyzes global policy frameworks. Additionally, a meta-analysis of per capita daily Fe and Zn intake from average wheat consumption was conducted. Notably, breeding-based approaches have led to the release of 40 biofortified wheat varieties for commercial cultivation in India, Pakistan, Bangladesh, Mexico, Bolivia, and Nepal, but this progress has overlooked Africa, a particularly vulnerable continent. Despite these advancements, there is a critical need for large-scale systematic investigations into the nutritional impact of biofortified wheat, indicating a crucial area for future research. This article can serve as a valuable resource for multidisciplinary researchers engaged in wheat biofortification, aiding in the refinement of ongoing and future strategies to achieve the Sustainable Development Goal of eradicating hunger and malnutrition by 2030.
[前沿资讯 ] Transcriptomic insights into the molecular mechanism for response of wild emmer wheat to stripe rust fungus 进入全文
Frontiers
A higher number of DEGs were identified in the moderately (Pst-resistant genotype, while the highly (Pst-resistant genotype exhibited a greater enrichment of pathways. Nonetheless, there were consistent patterns in the enrichment of pathways between the two genotypes at the same time of inoculation. At 24 hpi, a majority of pathways such as the biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, phenylalanine metabolism, and alpha-Linolenic acid metabolism exhibited significant enrichment in both genotypes. At 72 hpi, the biosynthesis of secondary metabolites and circadian rhythm-plant pathways were notably and consistently enriched in both genotypes. The majority of (WRKY, MADs , and AP2-ERF families were found to be involved in the initial stage of response to Pst invasion (24 hpi), while the MYB, NAC, TCP, and b-ZIP families played a role in defense during the later stage of Pst infection (72 hpi).
