Purification and immunoglobulin E epitopes identification of low molecular weight glutenin: an allergen in Chinese wheat
中国小麦过敏原低分子量麦谷蛋白的纯化及免疫球蛋白E表位鉴定
- 关键词：
- 来源：
- DOAJ
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2022-10-15
- 摘要：
- As one of the most important cereals, wheat (Triticum aestivum) has high nutritional value and is widely cultivated in China. However, wheat can cause severe allergic reactions, and a growing number of people are developing allergies to Chinese wheat. Low molecular weight glutenin (LMW-GS), an important allergen in susceptible populations, is responsible for celiac disease and wheat contacts dermatitis. In this study, LMW-GS was highly purified from Chinese wheat (Xiaoyan 6) and further identified and characterized. In addition, 8 peptides were predicted efficiently by 5 immunological tools, among which five peptides showed significant immunoglobulin E (IgE) binding abilities. Two specific epitopes were found to be in the non-conserved region of the amino acid sequence of LMW-GS, which was speculated to be the specific epitope of Chinese wheat. This systematic research of LMW-GS may provide new insights into the prevention of wheat allergy and development of hypoallergenic wheat products.
- 所属专题：
- 68
