In some embodiments of the present disclosure, method for isolation of Lantadene from Lantana Camara, are provided. Lantadene isolated according to these embodiments is checked for purity by melting point, UV spectroscopy, IR spectroscopy and HPLC. In different embodiments of the present disclosure, methods of detection of phenol in Lantadene by using electrode having immobilized enzyme, methods of detection of change in potential of Lantadene by using electrode having immobilized enzyme are provided. To fabricate electrodes used in the embodiments of the present disclosure, enzymes Monophenol monooxygenase and o-diphenol oxidase are entrapped in Abelmoschus escucentus: agar. Substrates used for Monophenol monooxygenase and o-diphenol oxidase are having phenolic groups. In some embodiments, these substances can be tyrosine or l-dopa.