Data from: A second-generation diagnostic single nucleotide polymorphism (SNP)-based assay, optimized to distinguish among eight poplar (Populus L.

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关键词：

Populus fremontii;Populus nigra;poplars;Populus maximowiczii;Populus trichocarpa;hybridization;Populus deltoides;clone certification;Populus angustifolia;species identification;Genotyping array;marker development;Populus;Populus laurifolia;Populus balsamifera

DOI：

doi:10.5061/dryad.sn577

摘要：

t of flanking and probe oligonucleotides was developed and tested. The accuracy of the SNP assay was validated by genotyping 448 putatively “pure” individuals

Data from: A set of plastid loci for use in multiplex fragment length genotyping for intraspecific variation in Pinus (Pinaceae)

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关键词：

Pinus resinosa;Pinus torreyana torreyana;Pinus taeda;Pinus albicaulis;discriminant analysis of principal components;Pinus cembroides;Pinus johannis;Pinus elliottii;Pinus heldreichii;Pinus rigida;Pinus massoniana;Pinus rzedowskii;Pinus ponderosa benthamiana;microsatellites;Pinus pseudostrobus var. apulcensis;Pinus bungeana;Pinus morrisonicola;Pinus chihuahuana;Pinus longaeva;Pinus Edulis;Pinus canariensis;Pinus leiophylla;Pinus attenuata;Pinus pinea;Pinus hartwegii subsp. donnell-smithii;Pinus montezumae;Pinus devoniana;Pinus lawsonii;Pinus pinaster;Pinus ponderosa var. benthamiana;Pinus douglasiana;Pinus yecorensis;plastid;Pinus arizonica;Pinus amamiana;Pinus pumila;Pinus kesiya;Pinus hartwegii;Pinus gerardiana;Pinus monticola;Pinus culminicola;Pinus muricata;Pinus sibirica;Pinus virginiana;Pinus oaxacana;Intraspecific Taxonomy;Pinus echinata;Pinus wallichiana;Pinus monophylla;Pinus serotina;Pinus densata;Pinus remota;Pinus caribea;Pinus squamata;Pinus dabeshanensis;Pinus strobus;Pinus pungens;Pinus taiwanensis;Pinus yunnanensis;Pinus clausa;Pinus koraiensis;Pinus pseudostrobus;Pinus nigra;Pinus densiflora;Pinus thunbergii;Pinus strobiformis;Pinus radiata;Pinus banksiana;Pinus dalatensis;Pinus jeffreyi;Pinus mugo;Pinus ponderosa scopulorum;Pinus palustris;Pinus ponderosa;Pinus glabra;Pinus ponderosa ponderosa;Pinus torreyana insularis;Pinus engelmannii;Pinus roxburghii;Pinus parviflora;Pinus tropicalis;Pinus cubensis;Pinus coulteri;Pinus lumholtzii;Pinus nelsonii;Pinus Krempfii;Pinus sabiniana;Pinus occidentalis;Pinus ponderos var. scopulorum;Pinus hwangshanensis;Pinus peuce;Pinus flexilis;Pinus contorta;Pinus sylvestris;Pinus ayacahuite;Pinus patula;Pinus pinceana;Pinus gregii;Pinus maximartinezii;Pinus quadrifolia;Pinus chiapensis;Pinus armandii;Pinus wangii;Pinus aristata;Pinus halepensis;Pinus cooperi;Pinus lambertiana;Pinus donnell smithii;Pinus pringlei;Pinus merkusii;Pinus brutia;Pinus discolor;Pinus latteri;Pinus fragilissima

DOI：

doi:10.5061/dryad.5nc25

摘要：

Premise of the study: Recently released Pinus plastome sequences support characterization of 15 plastid Simple Sequence Repeat (ptSSR) loci

Data from: Slippage of degenerate primers can cause variation in amplicon length

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关键词：

Metabarcoding;in silico

DOI：

doi:10.5061/dryad.nk81st2

摘要：

ould be taken to reduce slippage by improving protocols for primer design. For example, the flanking region adjacent to the 3? end of the primer