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Data from: Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea
- 负责人：
- DOI：
- doi:10.5061/dryad.1pm20
- 摘要：
- ng fish-specific 16S mitochondrial DNA primers, and sequenced using a metabarcoding workflow. DNA sequences were assigned to taxa using available gene
Data from: Elevated substitution rate estimates from ancient DNA: model violation and bias of Bayesian methods
- 负责人：
- DOI：
- doi:10.5061/dryad.1355
- 摘要：
- The increasing ability to extract and sequence DNA from non-contemporaneous tissue offers biologists the opportunity to analyze ancient DNA (aDNA
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
- 负责人：
- DOI：
- doi:10.5061/dryad.n4cv3
- 摘要：
- The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility
- 负责人：
- Porter, Teresita
- DOI：
- doi:10.5061/dryad.4m7d1
- 摘要：
- DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we asses
Data from: PrimerMiner: an R package for development and in silico validation of DNA metabarcoding primers
- 负责人：
- DOI：
- doi:10.5061/dryad.4c3g9
- 摘要：
- 1. DNA metabarcoding is a powerful tool to assess biodiversity by amplifying and sequencing a standardized gene marker region. Its success is oft
Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms
- 负责人：
- DOI：
- doi:10.5061/dryad.370nq
- 摘要：
- e compared first to a DNA reference library including the sequences of all the species used to constitute the mock community, and secondly to a complete DNA
Data from: Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach
- 负责人：
- 关键词：
- Eurtytemora affinis
- DOI：
- doi:10.5061/dryad.rh7f4
- 摘要：
- While standard DNA sequencing approaches readily yield genotypic sequence data, haplotype information is often of greater utility
Data from: Genotyping-in-Thousands by sequencing (GT-seq) panel development and application to minimally-invasive DNA samples to support studies
- 负责人：
- DOI：
- doi:10.5061/dryad.vr300p0
- 摘要：
- Minimally-invasive sampling (MIS) is widespread in wildlife studies; however, its utility for massively parallel DNA sequencing (MPS) is limited
Data from: Accelerating plant DNA barcode reference library construction using herbarium specimens: improved experimental techniques
- 负责人：
- DOI：
- doi:10.5061/dryad.s7282
- 摘要：
- in this study, DNA reconstruction and DNA-labelled next-generation sequencing can accelerate the construction of local reference libraries. By putting the local
Data from: From benchtop to desktop: important considerations when designing amplicon sequencing workflows
- 负责人：
- DOI：
- doi:10.5061/dryad.2qf0t
- 摘要：
- Amplicon sequencing has been the method of choice in many high-throughput DNA sequencing (HTS) applications. To date there has been a heavy focus
