Data from: High-throughput sequencing of nematode communities from total soil DNA extractions

负责人：

关键词：

Agriculture;nematode;soil;Nematoda

DOI：

doi:10.5061/dryad.0h653

摘要：

agricultural soils, we obtained 64.4 % sequences of nematode origin in total, whereas the remaining sequences were almost entirely metazoan. The nematode sequences wer

Data from: Automated size selection for short cell-free DNA fragments enriches for circulating tumor DNA and improves error correction duri

负责人：

关键词：

Next-generation sequencing;cell-free DNA;Melanoma;digital droplet PCR;pancreatic ductal adenocarcinoma;colorectal adenocarcinoma

DOI：

doi:10.5061/dryad.7pj51v0

摘要：

compared to the original ccfDNA yielded significantly larger family sizes (i.e., PCR duplicates) during in silico consensus sequence interpretation vi

Data from: Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA

负责人：

关键词：

mitogenome sequencing;Actinopterygii;eDNA

DOI：

doi:10.5061/dryad.q5gg0

摘要：

of fish species assembled from pooling genomic DNA extracted from tissues. In situ we utilized long-range PCR and Illumina sequencing to generate fragments bet

Data from: Fast and cost-effective genetic mapping in apple using next-generation sequencing

负责人：

关键词：

Malus x domestica

DOI：

doi:10.5061/dryad.55t54

摘要：

Next-generation DNA sequencing (NGS) produces vast amounts of DNA sequence data, but it is not specifically designed to generate data suitable

nchor: a novel approach for whole-genome comparative mapping utilising evolutionarily conserved DNA sequences"" data-category="" data-cropid="" data-dimen="" data-id="259A5B2C-B6B6-492D-834C-D41955585C5A"> Supporting data for "G-Anchor: a novel approach for whole-genome comparative mapping utilising evolutionarily conserved DNA sequences"

负责人：

关键词：

Genome-Mapping Metabolomic Software Workflow whole-genome alignment sequence mapping sequencing anchoring highly conserved elements (hce) genome evolution

DOI：

doi:10.5524/100415

摘要：

reference genome. G-Anchor makes novel use of a databank of highly conserved DNA sequence elements. We demonstrate how these elements may be aligned to a pair

Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera)

负责人：

关键词：

metagenomics;Late Pleistocene;Quaternary;Coleoptera;Insecta;shotgun sequencing;Carabidae;ancient DNA;Recent;DNA preservation;Wolbachia;museum DNA;Spiroplasma;Holocene;Amara alpina

DOI：

doi:10.5061/dryad.0179t

摘要：

r, the potential of these samples as genetic resources is currently unassessed. Here, using Sanger and Illumina shotgun sequence data, we explored DNA prese

Data from: Tag jumps illuminated – reducing sequence-to-sample misidentifications in metabarcoding studies

负责人：

关键词：

Environmental DNA;Metabarcoding;Tag jumping;Diversity assessment;Second generation sequencing;Chimeras

DOI：

doi:10.5061/dryad.64687

摘要：

, we address the problem of sequences in metabarcoding sequencing outputs with false combinations of used tags (tag jumps). Unless these sequences

cytochrome b<\/i> gene sequence

摘要：

(cytb) gene sequences of mammals were identified. The results of a sequence identity test indicated that those sequences were derived from four rodent

Data from: Evolutionary patterns and processes: lessons from ancient DNA

负责人：

关键词：

Temporal sampling;palaeogenomics;metagenomics;population genomics;ancient DNA

DOI：

doi:10.5061/dryad.tt78r

摘要：

in the fossil record. With the recent development of high-throughput DNA sequencing technologies and molecular techniques tailored to ultra-damaged templates, it ha

Data from: A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3,500 identified species

负责人：

关键词：

DNA Barcoding Ecosystem Services

DOI：

doi:10.5061/dryad.gg8fg

摘要：

regional test of the efficiency of DNA barcoding for beetles with a focus on Germany. Sequences ≥500 bp were recovered from 63% of the specimens analysed (15 948