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Understanding the factors conditioning fall armyworm (Spodoptera frugiperda J.E. Smith) infestation in African smallholder maize fields and quantifying its impact on yield. A case study in Eastern Zimbabwe.
- 负责人：
- Baudron, Frédéric
- 关键词：
- DOI：
- doi:10.7910/dvn/yykx1t
- 摘要：
- . In Chipinge District, Wards 16, 18 and 20 were selected as the drier, intermediate and wetter wards, respectively. In Makoni District, Wards 26, 28 and 34 wer
Data from: Planktonic protistan communities in lakes along a large-scale environmental gradient
- 负责人：
- DOI：
- doi:10.5061/dryad.7s6s8
- 摘要：
- . We applied 18S rDNA amplicon sequencing to provide a comprehensive analysis of eukaryotic diversity in 74 low-productivity lakes along a 750 km longitudinal
Data from: Plant diversity and density predict belowground diversity and function in an early successional alpine ecosystem
- 负责人：
- DOI：
- doi:10.5061/dryad.tn39dc6
- 摘要：
- , fungal and eukaryotic species richness and phylogenetic diversity (using 16S, ITS, and 18S gene sequencing), and ecosystem function (levels of soil C
Data from: Metagenetic community analysis of microbial eukaryotes illuminates biogeographic patterns in deep-sea and shallow water sediments
- 负责人：
- DOI：
- doi:10.5061/dryad.vd094
- 摘要：
- Microbial eukaryotes (nematodes, protists, fungi, etc., loosely referred to as meiofauna) are ubiquitous in marine sediments and likely play pivotal roles in maintaining ecosystem function. Although the deep-sea benthos represents one of the world’s largest habitats, we lack a firm understanding of the biodiversity and community interactions amongst meiobenthic organisms in this ecosystem. Within this vast environment key questions concerning the historical genetic structure of species remain a mystery, yet have profound implications for our understanding of global biodiversity and how we perceive and mitigate the impact of environmental change and anthropogenic disturbance. Using a metagenetic approach, we present an intensive assessment of microbial eukaryote communities across depth gradients (shallow water to abyssal) and ocean basins (deep-sea Pacific and Atlantic). Our results show that while some taxa can maintain eurybathic ranges and cosmopolitan deep-sea distributions, the majority of species appear to be regionally restricted in marine habitats. For OCTUs reporting wide distributions, there appears to be a taxonomic bias towards a small subset of taxa in most phyla; such bias may be driven by specific life history traits amongst these organisms. In addition, low genetic divergence between geographically disparate deep-sea sites suggests either a shorter coalescence time between deep-sea regions or slower rates of evolution across this vast oceanic ecosystem. While high-throughput studies allow for broad assessment of genetic patterns across microbial eukaryote communities, intragenomic variation in rRNA gene copies and the patchy coverage of reference databases currently present substantial challenges for robust taxonomic interpretations of eukaryotic datasets.
Data from: Coral feeding on microalgae assessed with molecular trophic markers
- 负责人：
- DOI：
- doi:10.5061/dryad.3kf75
- 摘要：
- and Oculina arbuscula. Herbivory was assessed by end-point PCR amplification of algae-specific 18S rRNA gene fragments purified from coral tissue genomic DNA
Data from: Metabarcoding reveals environmental factors influencing spatio-temporal variation in pelagic micro-eukaryotes
- 负责人：
- DOI：
- doi:10.5061/dryad.442dv
- 摘要：
- Marine environments harbour a vast diversity of micro-eukaryotic organisms (protists and other small eukaryotes) that play important roles in structuring marine ecosystems. However, micro-eukaryote diversity is not well understood. Likewise, knowledge is limited regarding micro-eukaryote spatial and seasonal distribution, especially over long temporal scales. Given the importance of this group for mobilizing energy from lower trophic levels near the base of the food chain to larger organisms, assessing community stability, diversity and resilience is important to understand ecosystem health. Herein, we use a metabarcoding approach to examine pelagic micro-eukaryote communities over a 2.5-year time series. Bimonthly surface sampling (July 2009 to December 2011) was conducted at four locations within Mobile Bay (Bay) and along the Alabama continental shelf (Shelf). Alpha-diversity only showed significant differences in Shelf sites, with the greatest differences observed between summer and winter. Beta-diversity showed significant differences in community composition in relation to season and the Bay was dominated by diatoms, while the Shelf was characterized by dinoflagellates and copepods. The northern Gulf of Mexico is heavily influenced by the Mobile River Basin, which brings low-salinity nutrient-rich water mostly during winter and spring. Community composition was correlated with salinity, temperature and dissolved silicate. However, species interactions (e.g. predation and parasitism) may also contribute to the observed variation, especially on the Shelf, which warrants further exploration. Metabarcoding revealed clear patterns in surface pelagic micro-eukaryote communities that were consistent over multiple years, demonstrating how these techniques could be greatly beneficial to ecological monitoring and management over temporal scales.
Data from: Locality or habitat? Exploring predictors of biodiversity in Amazonia
- 负责人：
- DOI：
- doi:10.5061/dryad.4hv2b38
- 摘要：
- and 18S gene sequences, respectively. We characterize richness and community composition based on operational taxonomic units (OTUs) and test thei
Data from: Morphological and molecular diagnostic species characters of Staurozoa (Cnidaria) collected on the coast of Helgoland (German Bight, North Sea)
- 负责人：
- DOI：
- doi:10.5061/dryad.5cr6008
- 摘要：
- of mitochondrial 16S and COI and nuclear 18S ribosomal DNA identified the three species and confirmed their morphological identification. In comparison to published
Data from: Description of two new species of Indo-Pacific Thylacoplethus and a new record of Thompsonia japonica (Rhizocephala: Akentrogonida: Thompsoniidae) from hermit, porcelain and mud crabs (Decapoda) based on morphological and molecular data
- 负责人：
- DOI：
- doi:10.5061/dryad.6s7p8
- 摘要：
- based on morphological characters and DNA sequences of the nuclear 18S rDNA gene. The new species from the porcellanid crab comprises the first record
Data from: Deep-sea, deep-sequencing: metabarcoding extracellular DNA from sediments of marine canyons
- 负责人：
- DOI：
- doi:10.5061/dryad.520gq
- 摘要：
- ng an environmental DNA metabarcoding. We used a new primer set targeting a short eukaryotic 18S sequence (ca. 110 bp). We applied a protocol designed to obtain extractions
