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Data from: Neonatal activation of the xenobiotic-sensors PXR and CAR results in acute and persistent down-regulation of PPAR?-signaling in mouse liver
- 负责人:
- DOI:
- doi:10.5061/dryad.0k90t
- 摘要:
- gene loci. RT-qPCR, western blotting, and enzyme activity assays in livers of wild-type, PXR-null, and CAR-null mice confirmed that the persistent
Data from: Environmental DNA (eDNA) detects the invasive rusty crayfish (Orconectes rusticus) at low abundances
- 负责人:
- DOI:
- doi:10.5061/dryad.3hp15
- 摘要:
- conventional baited trapping as a measure of crayfish relative abundance with water samples for eDNA, which were analysed in the laboratory with a qPCR
Data from: A metatranscriptomic analysis of diseased social wasps (Vespula vulgaris) for pathogens, with an experimental infection of larvae
- 负责人:
- DOI:
- doi:10.5061/dryad.m98pt3k
- 摘要:
- t we monitored, however, had a significantly higher prevalence in diseased than in healthy larvae. RT-qPCR analysis indicated that pathogen infections were signi
Data from: Seasonal dynamics and co-occurrence patterns of honey bee pathogens revealed by high-throughput RT-qPCR analysis
- 负责人:
- DOI:
- doi:10.5061/dryad.q7s60q9
- 摘要:
- s for a high-throughput qPCR platform. Assuming that interaction of pathogens requires co-occurrence within the same individual, single worker bees were analyzed
Data from: Extracting DNA from ‘jaws’: high yield and quality from archived tiger shark (Galeocerdo cuvier) skeletal material
- 负责人:
- DOI:
- doi:10.5061/dryad.8080c
- 摘要:
- s (Galeocerdo cuvier). Protocols were compared for DNA yield and quality using a qPCR approach. For jaw swarf, all methods provided relatively high DNA yield and quality
Data from: Early replication dynamics of sex-linked mitochondrial DNAs in the doubly uniparental inheritance species Ruditapes philippinarum (Bivalvia Veneridae)
- 负责人:
- DOI:
- doi:10.5061/dryad.q3hs1
- 摘要:
- ng real-time quantitative PCR. We found that both mtDNAs do not detectably replicate during early embryogenesis, and that the M line might be lost from females around 24?h of age
Data from: A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater
- 负责人:
- 关键词:
- DOI:
- doi:10.5061/dryad.2fqz612km
- 摘要:
- .? Flow cytometry, amplicon sequencing?of?16S and 18S rRNA genes and micro-fluidic quantitative polymerase-chain reactions (MFQPCR) were used to determine chlorophyll
Data from: Multiple pairwise analysis of non-homologous centromere coupling reveals preferential chromosome size-dependent interactions and a role for bouquet formation in establishing the interaction pattern
- 负责人:
- DOI:
- doi:10.5061/dryad.71425
- 摘要:
- ible interactions between non-homologous yeast centromeres during early meiosis. Using this variant of 3C-qPCR, we found a size-dependent interaction
Data from: Stress hormone receptors change as range expansion progresses in house sparrows
- 负责人:
- DOI:
- doi:10.5061/dryad.8pq6c
- 摘要:
- As ranges expand, individuals encounter different environments at the periphery than at the centre of the range. Previously, we have shown that glucocorticoids (GCs) vary with range expansion: individuals at the range edge release more GCs in response to restraint. Here, we measured hippocampal mRNA expression of GC receptors (mineralocorticoid, MR and glucocorticoid, GR) in eight house sparrow (Passer domesticus) populations varying in age. We found that individuals closest to the range edge had the lowest expression of MR relative to GR; in all likelihood, this relationship was driven by a marginal reduction of MR mRNA at the range edge. Reduced MR (relative to GR) might allow enhanced GC binding to GR, the lower affinity receptor that would enhance a rapid physiological and behavioural response to stressors. The insights gained from this study are not only enlightening to introduced species, but may also predict how certain species will react as their ranges shift owing to anthropogenic changes.
Data from: Individual consumption of supplemental food as a predictor of reproductive performance and viral infection intensity
- 负责人:
- DOI:
- doi:10.5061/dryad.t7s15hp
- 摘要:
- d stable isotope analyses to characterise the relative proportional consumption of supplemental food and quantitative polymerase chain reaction (qPCR) to assess beak