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Data from: Advanced characterization of DNA molecules in rAAV vector preparations by single-stranded virus next-generation sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.fs4cp
- 摘要：
- studies using quantitative polymerase chain reaction (qPCR) analyses. However, because qPCR only provides a partial view of the DNA molecules in rAAV
Data from: Environmental DNA analysis of river herring in Chesapeake Bay: a powerful tool for monitoring threatened keystone species
- 负责人：
- DOI：
- doi:10.5061/dryad.n60t8b3
- 摘要：
- e developed and field-tested a qPCR assay to detect eDNA from alewife and blueback herring (collectively known as ‘river herring’), comparing eDNA-based
Data from: Recognizing false positives: synthetic oligonucleotide controls for environmental DNA surveillance
- 负责人：
- DOI：
- doi:10.5061/dryad.h373j
- 摘要：
- . For quantitative PCR (qPCR), false positives in environmental samples can also be detected using a fluorescent probe designed to detect the synthetic insert. The gene
Data from: High Rates of Asymptomatic, Sub-microscopic Plasmodium vivax Infection and Disappearing Plasmodium falciparum Malaria in an Area of Low
- 负责人：
- DOI：
- doi:10.5061/dryad.545tv
- 摘要：
- by quantitative PCR (qPCR) and light microscopy (LM). Presence of gametocytes was determined by reverse transcription quantitative PCR (RT-qPCR). Results: By qPCR, 468
Data from: Factors influencing detection of eDNA from a stream-dwelling amphibian
- 负责人：
- DOI：
- doi:10.5061/dryad.mt60p
- 摘要：
- ng stream-dwelling salamanders and quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (1) production rate, (2) persistence time
Data from: Gene transcription in sea otters (Enhydra lutris): development of a diagnostic tool for sea otter and ecosystem health
- 负责人：
- DOI：
- doi:10.5061/dryad.1nn7v
- 摘要：
- natural variation. Herein we describe methodology for the development of quantitative real time polymerase chain reaction (qPCR) assays to measure differential
Data from: Haemosporidian prevalence and parasitemia in the tufted titmouse (Baeolophus bicolor)
- 负责人：
- DOI：
- doi:10.5061/dryad.rd927
- 摘要：
- oscopy, polymerase chain reaction (PCR), and quantitative PCR (qPCR). Thirteen mitochondrial cytochrome b lineages of haemosporidian parasites were found including
Data from: Diagnostic gene expression biomarkers of coral thermal stress
- 负责人：
- 关键词：
- qPCR;Porites astreoides;cellular stress response;double-gene assay;cellular homeostasis response
- DOI：
- doi:10.5061/dryad.1h0n1
- 摘要：
- erested in linking organism physiology with large-scale climatic conditions. Here, we assessed the ability of quantitative PCR (qPCR) based gene expression biomarkers
Data from: DNA extraction method affects the detection of a fungal pathogen in formalin-fixed specimens using qPCR
- 负责人：
- 关键词：
- frogs amphibians amphibian decline natural history collections disease chytridiomycosis DNA extraction
- DOI：
- doi:10.5061/dryad.060tg
- 摘要：
- preserved specimens for Bd can improve our understanding of its emergence and spread. Quantitative PCR (qPCR) enables Bd detection with minimal disturbance
Data from: Long-term endemism of two highly divergent lineages of the amphibian-killing fungus in the Atlantic Forest of Brazil
- 负责人：
- DOI：
- doi:10.5061/dryad.m994g
- 摘要：
- and origin of Bd. Focusing on the Atlantic Forest (AF) of Brazil, we used qPCR assays to determine the presence or absence of Bd on 2799 preserved
