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Data from: Calcium imaging with genetically encoded sensor Case12: facile analysis of ?7/?9 nAChR mutants
负责人:
关键词:
Naja kaouthia;Xenopus laevis
DOI:
doi:10.5061/dryad.4k831
摘要:
ed light on their involvement in different physiological functions and diseases. Combination of site-directed mutagenesis and electrophysiology is a powerful
Data from: Trans-toxin ion-sensitivity of charybdotoxin-blocked potassium-channels reveals unbinding transitional states
负责人:
关键词:
protein unbinding;Shaker Kv-channels;Charybdotoxin;Xenopus;Transition State;protein-protein interaction;protein wobbling
DOI:
doi:10.5061/dryad.0p77qk4
摘要:
r dissociation are unclear. Protein-protein complexes, lasting from microseconds to years, often involve induced-fit, challenging computational or kinetic analysis
Data from: A robust and tunable mitotic oscillator in artificial cells
负责人:
关键词:
DOI:
doi:10.5061/dryad.2929bs2
摘要:
Xenopus egg extracts in water-in-oil microemulsions, we developed artificial cells that are adjustable in sizes and periods, sustain mitotic oscillations for over 30
MOESM1 of Adaption of a dermal in vitro method to investigate the uptake of chemicals across amphibian skin
负责人:
关键词:
Biochemistry Space Science Physiology Pharmacology 59999 Environmental Sciences not elsewhere classified 39999 Chemical Sciences not elsewhere classified Ecology Immunology 69999 Biological Sciences not elsewhere classified Developmental Biology
DOI:
doi:10.6084/m9.figshare.c.3623135_d4.v1
摘要:
g to treatment (test compound and storage); Xlf = Xenopus laevis, female; used animals are numbered consecutively (2\u20137); skin side is described as d = dorsal
MOESM1 of Adaption of a dermal in vitro method to investigate the uptake of chemicals across amphibian skin
负责人:
关键词:
Biochemistry Space Science Physiology Pharmacology 59999 Environmental Sciences not elsewhere classified 39999 Chemical Sciences not elsewhere classified Ecology Immunology 69999 Biological Sciences not elsewhere classified Developmental Biology
DOI:
doi:10.6084/m9.figshare.c.3623135_d4
摘要:
g to treatment (test compound and storage); Xlf = Xenopus laevis, female; used animals are numbered consecutively (2\u20137); skin side is described as d = dorsal
Data from: A unified anatomy ontology of the vertebrate skeletal system
负责人:
关键词:
Phenotype;skeleton;Vertebrata;reasoning;Integration;semantics;comparative morphology;ontology
DOI:
doi:10.5061/dryad.6bt92
摘要:
the existing skeletal terminologies for the species-specific (mouse, the frog Xenopus, zebrafish) and multispecies (teleost, amphibian) vertebrate anatomy
Data from: High-throughput identification of informative nuclear loci for shallow-scale phylogenetics and phylogeography
负责人:
关键词:
Pseudacris;Hyla;Next-generation sequencing;Acris;phylogenetics;reduced-representation library;Illumina HiSeq;anonymous nuclear markers;phylogeography
DOI:
doi:10.5061/dryad.vh151q1c
摘要:
(Xenopus tropicalis) to generate expectations for the number of loci and degree of coverage for a particular experimental design. Using the RRL approach
Data from: Molecular cytogenetic and genomic insights to chromosomal evolution
负责人:
关键词:
Placentalia;Mesozoic;Amniota;ancestral karyotypes;Paleocene;Eocene;Cretaceous;comparative cytogenetics;syntenic segmental associations;conserved syntenies;Mammalia;Fish;Xenopus tropicalis;Monodelphis domestica;phylogenomics;Tetrapoda;Gallus gallus
DOI:
doi:10.5061/dryad.7j0b8468
摘要:
This review summarizes aspects of the extensive literature on the patterns and processes underpinning chromosomal evolution in vertebrates and especially placental mammals. It highlights the growing synergy between molecular cytogenetics and comparative genomics, particularly with respect to fully or partially sequenced genomes, and provides novel insights into changes in chromosome number and structure across deep division of the vertebrate tree of life. The examination of basal numbers in the deeper branches of the vertebrate tree suggest a haploid (n) chromosome number of 10–13 in an ancestral vertebrate, with modest increases in tetrapods and amniotes most probably by chromosomal fissioning. Information drawn largely from cross-species chromosome painting in the data-dense Placentalia permits the confident reconstruction of an ancestral karyotype comprising n=23 chromosomes that is similarly retained in Boreoeutheria. Using in silico genome-wide scans that include the newly released frog genome we show that of the nine ancient syntenies detected in conserved karyotypes of extant placentals (thought likely to reflect the structure of ancestral chromosomes), the human syntenic segmental associations 3p/21, 4pq/8p, 7a/16p, 14/15, 12qt/22q and 12pq/22qt predate the divergence of tetrapods. These findings underscore the enhanced quality of ancestral reconstructions based on the integrative molecular cytogenetic and comparative genomic approaches that collectively highlight a pattern of conserved syntenic associations that extends back ~360 million years ago.

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