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Data from: TERAD: Extraction of transposable element composition from RADseq data
负责人:
关键词:
Synalpheus;bioinformatics;mobile genetic element;genome size;ddRADseq
DOI:
doi:10.5061/dryad.2375033
摘要:
ge whole genome sequencing are comparable within and across species. Finally, we develop a new bioinformatic pipeline, TERAD, to extract TE compositions from RADseq data
Data from: Influence of killing method on Lepidoptera DNA barcode recovery
负责人:
关键词:
Recent;freezing;killing method;Lepidoptera;ethyl acetate;cyanide
DOI:
doi:10.5061/dryad.18b1k
摘要:
in good condition for later study. Factors such as time since collection, correct storage (exposure to free water and heat) and DNA extraction protocol ar
Data from: Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter
负责人:
关键词:
filter capsule;Environmental DNA;qPCR;eDNA
DOI:
doi:10.5061/dryad.p2q4r
摘要:
ge and extraction techniques of eDNA. Previous comparative studies for eDNA capture/storage have tested precipitation and ‘open’ filters. However, practical ‘enclosed’ filters
Data from: Museum genomics: low-cost and high-accuracy genetic data from historical specimens
负责人:
关键词:
historical DNA;Next-generation sequencing;Rattus;Rattus norvegicus;natural history collections;Holocene
DOI:
doi:10.5061/dryad.1fm3f
摘要:
an opportunity to uncover much of this variation; however, genetic studies of historical museum specimens typically rely on extracting highly degra
Data from: Fit for genomic and proteomic purposes: sampling the fitness of nucleic acid and protein derivatives from formalin fixed paraffin embedded
负责人:
关键词:
DOI:
doi:10.5061/dryad.qc1jn
摘要:
in extraction and purification methods, making these derivatives available for numerous genomic and proteomic platforms. Previously, DNA, RNA, microRNA (miRNA), or protein derived
Data from: Intraspecific DNA contamination distorts subtle population structure in a marine fish: decontamination of herring samples
负责人:
关键词:
population genetics;Heterozygosity;DNA contamination;Pacific herring;Clupea pallasii;RAD sequencing
DOI:
doi:10.5061/dryad.g28rh86
摘要:
minutes in bleach or left untreated, and extracted DNA was used to prepare DNA libraries using a restriction-site associated DNA (RAD) approach. Our result
Data from: A sequel to Sanger: amplicon sequencing that scales
负责人:
关键词:
nucleotide composition;SMRT sequencing;Nuclear DNA;homopolymer;Mitochondrial DNA;DNA Barcoding;phylogenetics;PCR
DOI:
doi:10.5061/dryad.4368h
摘要:
Sanger analysis. Reflecting the capacity of each instrument to recover sequences from more than five million DNA extracts a year, this platform fac
Data from: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: testing multiple eDNA workflows.
负责人:
关键词:
Misgurnus fossilis;eDNA sampling
DOI:
doi:10.5061/dryad.4xgxd256h
摘要:
combinations for long-term sample preservation, DNA capture and DNA extraction. Additionally, we analyzed the DNA yield of samples from a ditch with a natura
Data from: “Direct PCR” optimization yields a rapid, cost-effective, non-destructive, and efficient method for obtaining DNA barcodes without DNA extraction
负责人:
关键词:
ecosystem services;Chironomidae;DNA Barcoding;insects;Invertebrates
DOI:
doi:10.5061/dryad.4q4kf
摘要:
ve PCR method that skips DNA extraction (“direct PCR”) and that can be used for a broad range of invertebrate taxa. We demonstrate how direct PCR can be opti

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