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Data from: Finding flies in the mushroom soup: host specificity of fungus-associated communities revisited with a novel molecular method
负责人:
关键词:
Fungi;community structure;Ecological interaction networks;Metabarcoding;DNA extraction and purification;Host specificity
DOI:
doi:10.5061/dryad.tf56fm1
摘要:
convenient complements. To overcome challenges in DNA extraction and PCR associated with the complex chemical properties fruiting bodies, we developed
Data from: High-throughput genotyping of Anopheles mosquitoes using intact legs by Agena Biosciences iPLEX
负责人:
关键词:
DNA extraction;genotyping;Anopheles gambiae;Anopheles arabiensis;Ecological Genetics;Population Genetics - Empirical
DOI:
doi:10.5061/dryad.nr716
摘要:
the opportunity to develop more effective genotyping strategies for high-throughput screening. A major bottleneck of this goal is nucleic acid extraction. Her
Data from: Tracking earthworm communities from soil DNA
负责人:
Taberlet, Pierre
关键词:
Community Ecology DNA Barcoding Invertebrates Population Ecology DNA metabarcoding
DOI:
doi:10.5061/dryad.3mv8v434
摘要:
of ecosystem health. To date, earthworm diversity has been investigated through conventional extraction methods such as handsorting, soil washing or the applicati
Data from: Development and validation of rapid environmental DNA (eDNA) detection methods for bog turtle (Glyptemys muhlenbergii)
负责人:
关键词:
bog turtle;C. elegans;DNA extraction;Environmental DNA;internal control;Glyptemys muhlenbergii;sample interference;qPCR inhibition;eDNA;DNA recovery
DOI:
doi:10.5061/dryad.6n7675r
摘要:
DNA extraction methods, limit false negative results due to qPCR inhibition, and measure total DNA recovery from each sample. Using the internal control, we found that DNA
Data from: Automated size selection for short cell-free DNA fragments enriches for circulating tumor DNA and improves error correction duri
负责人:
关键词:
Next-generation sequencing;cell-free DNA;Melanoma;digital droplet PCR;pancreatic ductal adenocarcinoma;colorectal adenocarcinoma
DOI:
doi:10.5061/dryad.7pj51v0
摘要:
on in the original ccfDNA was commonly lost at family sizes necessary to minimize errors. These collective findings support the automated extraction of short ccfDNA fra
Data from: DNA-based analysis of regurgitates: a noninvasive approach to examine the diet of invertebrate consumers
负责人:
Traugott, Michael
关键词:
Predator Prey Interactions Invertebrates Insects Community Ecology
DOI:
doi:10.5061/dryad.62hk145k
摘要:
ture prior to DNA extraction. We conclude that in those invertebrates where regurgitates can be obtained, examination of food DNA in regurgitates offers ma
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
负责人:
关键词:
moss;diversity;Peltigera praetextata;Brachythecium fendleri;454 amplicon pyrosequencing;ITSrDNA;lichen;Pinus leiophylla var. chihuahuana;mycobiont
DOI:
doi:10.5061/dryad.8271f
摘要:
ons, and composition. The aim of this study was to examine how tissue storage prior to DNA extraction, primer design, and various quality-control approaches commonly use
Data from: Preservation-induced morphological change in salamanders and failed DNA extraction from a decades-old museum specimen: implicati
负责人:
关键词:
DOI:
doi:10.5061/dryad.63xsj3tzd
摘要:
DNA extraction techniques on the putatively formalin-fixed P. ainsworthi holotype. We used Bayesian models to demonstrate that preservati

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