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Data from: Genome-wide single nucleotide polymorphism (SNP) identification and characterization in a non-model organism, the Africa
- 负责人：
- DOI：
- doi:10.5061/dryad.q8q11
- 摘要：
- of putative SNPs that passed the filtering criterion. Our set of SNPs was validated by PCR amplification and Sanger sequencing of 15 SNPs. Of these 15 SNPs, 14
Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- on of non-specific amplification effects than other mismatches. To conclude, we show that prediction of primer specificity based only on the number and positi
Data from: Multiplex PCR targeting lineage specific SNPs ? A highly efficient and simple approach to block out predator sequences in molecular
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.44mn451
- 摘要：
- , we use predator lineage-specific SNPs at the 3’-end of PCR primers to selectively block out predators from amplification. While this approach lar
Data from: Estimating synchronous demographic changes across populations using hABC and its application for a herpetological community from
- 负责人：
- DOI：
- doi:10.5061/dryad.789pv
- 摘要：
- the influence of Quaternary climate on diversification processes. Hierarchical approximate Bayesian computation (hABC) is a powerful and flexible approach tha
Data from: High-throughput microsatellite genotyping in ecology: improved accuracy, efficiency, standardization and success with low-quanti
- 负责人：
- DOI：
- doi:10.5061/dryad.18tg7
- 摘要：
- on the continued use of microsatellites for certain applications. We present a study for improving microsatellite genotyping in ecology usi
Data from: Genetic identification of Iberian rodent species using both mitochondrial and nuclear loci: application to non-invasive sampling
- 负责人：
- Alves, Paulo C.
- DOI：
- doi:10.5061/dryad.fv21h
- 摘要：
- . Amplification success for the cyt-b and IRBP fragment was 85% and 43% in faecal and 88% and 64% in owl-pellet DNA extractions, respectively. The method allows
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
- 负责人：
- DOI：
- doi:10.5061/dryad.8271f
- 摘要：
- in a model data set. Third, we show that inferences regarding endolichenic diversity can be improved by using a novel primer that reduces amplification
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan
- 负责人：
- DOI：
- doi:10.5061/dryad.443t4m1q
- 摘要：
- bp of the mitochondrial 12S rRNA gene, with and without a blocking oligonucleotide specific to the predator sequence. The amplification products wer
Data from: No ecological opportunity signal on a continental scale? Diversification and life-history evolution of African true toads (Anura
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.21m3t
- 摘要：
- , the diversification processes might be more complex. Thirdly, lower speciation rates of habitat generalists may have affected overall proliferation of lineages.
Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods
- 负责人：
- DOI：
- doi:10.5061/dryad.2p51n
- 摘要：
- t (up to five orders of magnitude) on the amplification efficiency. In particular, the number of primer–template mismatches explained most of the variati
