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Data from: The choice of universal primers and the characteristics of the species mixture determines when DNA metabarcoding can be quantitative.
- 负责人：
- DOI：
- doi:10.5061/dryad.q2r3b1f
- 摘要：
- t modulate amplification efficiency, we focused on the number of primer-template mismatches, arguably the most important one. We used 15 common primers pairs
Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores
- 负责人：
- DOI：
- doi:10.5061/dryad.23k27
- 摘要：
- mitochondrial (mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA samples under natural field conditions for 20 fresh scats/species/season from
Data from: A multivariate test of disease risk reveals conditions leading to disease amplification
- 负责人：
- DOI：
- doi:10.5061/dryad.5tj50
- 摘要：
- others. Yet, few empirical studies demonstrate amplification. This contrast may occur because few studies have considered the multivariate nature
Data from: Browsed twig environmental DNA: diagnostic PCR to identify ungulate species
- 负责人：
- Nichols, Ruth V.
- 关键词：
- Ecological Genetics Mammals Species Interactions Wildlife Management Foraging Patterns Forest Ecosystems
- DOI：
- doi:10.5061/dryad.3nh92
- 摘要：
- (Capreolus capreolus), fallow deer (Cervus dama), and red deer (Cervus elaphus) are sympatric, amplification success reached 75%. This method promises to greatly improve
Data from: ITS1: a DNA barcode better than ITS2 in eukaryotes?
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n56t9
- 摘要：
- three aspects: PCR amplification, DNA sequencing and species discrimination, in terms of the presence of DNA barcoding gaps, species discrimination efficiency
Data from: Muscle tradeoffs in a power-amplified prey capture system
- 负责人：
- DOI：
- doi:10.5061/dryad.t91v2
- 摘要：
- amplification, not direct lever action. Power-amplified systems typically use slow, forceful muscles to pre-load springs which then rapidly release
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
- 负责人：
- DOI：
- doi:10.5061/dryad.n4cv3
- 摘要：
- are widely applied to the ITS region without verification of their performance. We examined the rate of chimera formation during amplification
Data from: Partitioning between atmospheric deposition and canopy microbial nitrification into throughfall nitrate fluxes in a Mediterranean forest
- 负责人：
- DOI：
- doi:10.5061/dryad.3cq6127
- 摘要：
- s highlight the power of coupling microbial community analysis, functional gene amplification and stable isotope approaches to examine ecosystem-scale processes.
Data from: Evaluating DNA degradation rates in faecal pellets of the endangered pygmy rabbit
- 负责人：
- 关键词：
- Brachylagus idahoensis;pygmy rabbit;genotyping error;noninvasive genetic sampling;DNA degradation
- DOI：
- doi:10.5061/dryad.722qb
- 摘要：
- for microsatellite loci declined rapidly from 80.0% to 42.7% between days 5 and 7, likely due to increased environmental temperature. Success rates for mtDNA amplification
Data from: De novo discovery and multiplexed amplification of microsatellite markers for black alder (Alnus glutinosa) and related species using SSR
- 负责人：
- DOI：
- doi:10.5061/dryad.gg0jm
- 摘要：
- e tested for amplification, twelve of which were polymorphic in A. glutinosa. These twelve loci were successfully co-amplified in a single multi
