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Data from: Previously unknown evolutionary groups dominate the ssDNA gokushoviruses in oxic and anoxic waters of a coastal marine environment
- 负责人：
- DOI：
- doi:10.5061/dryad.7gg25
- 摘要：
- asonally anoxic fjord on the coast of Vancouver Island, British Columbia. Amplicon sequencing of samples from the mixed oxic surface (10 m) and deeper anoxic (200
Data from: Analysis of microsatellite loci in tree of heaven (Ailanthus altissima (Mill.) Swingle) using SSR-GBS
- 负责人：
- 关键词：
- genotyping by sequencing;SNP;microsatellite;Amplicon Sequencing;Ailanthus altissima;Tree of heaven
- DOI：
- doi:10.5061/dryad.873b2j2
- 摘要：
- amplicon sequencing (SSR-GBS). We screened three populations from Eastern Austria for genetic variation at 19 newly identified microsatellite loci. We tested two diffe
Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
- 负责人：
- DOI：
- doi:10.5061/dryad.fs728
- 摘要：
- number or (4) avoiding locus specific amplification by directly sequencing genomic DNA. Amplification bias is reduced considerably by degenerate primers
Data from: Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns
- 负责人：
- Bunce, Michael
- DOI：
- doi:10.5061/dryad.8ps58rp2
- 摘要：
- gene over 50,000 amplicon sequence reads were generated from 15 TCM samples presented in the form of powders, tablets, capsules, bile flakes and herbal teas
Data from: Parallel tagged amplicon sequencing reveals major lineages and phylogenetic structure in the North American tiger salamander
- 负责人：
- DOI：
- doi:10.5061/dryad.03s86
- 摘要：
- loci are analyzed. We present the results of an initial application of parallel tagged sequencing (PTS) on a next generation platform to sequence
amplicon sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.83gf113b
- 摘要：
- 454 sequencing and developed a bioinformatic pipeline to process multiplexed tagged amplicons in order to identify single nucleotide polymorphisms and allelic
Data from: The importance of validating the demethylating effect of 5-aza-dC in model species
- 负责人：
- DOI：
- doi:10.5061/dryad.d305qm1
- 摘要：
- its effectiveness. We used bisulfite amplicon sequencing of ten genes that were either heavily methylated, previously showed a response to 5-aza-dC or suggested
Data from: Large-scale genotyping of highly polymorphic loci by next generation sequencing: how to overcome the challenges to reliably genotype individuals?
- 负责人：
- DOI：
- doi:10.5061/dryad.rp7n9
- 摘要：
- ers of individuals. Next-generation sequencing (NGS) techniques enable such large-scale sequencing, but stringent data processing is required. Here, we develop an easy
Data from: Parallel tagged amplicon sequencing of transcriptome-based genetic markers for Triturus newts with the Ion Torrent
- 负责人：
- DOI：
- doi:10.5061/dryad.36775
- 摘要：
- and sequenced on the Ion Torrent next-generation sequencing platform. A bioinformatics pipeline identifies the alleles and recodes these to a genotypic format. Ne
Data from: Anchored hybrid enrichment for massively high-throughput phylogenomics
- 负责人：
- DOI：
- doi:10.5061/dryad.r606d128
- 摘要：
- of traditional Sanger sequencing and ~3.5% of the cost of high-throughput amplicon sequencing for projects on the scale of 500 loci x 100 individuals) and r
