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Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
负责人:
关键词:
cloning\/sanger sequencing;ITS1 region;Caullerya mesnili;Amplicon Sequencing
DOI:
doi:10.5061/dryad.v7f7r
摘要:
whether the estimated frequency of representative sequences, generated by 454 amplicon sequencing, differs significantly from that obtaine
Data from: Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)
负责人:
关键词:
DOI:
doi:10.5061/dryad.n8v4v6d
摘要:
for amplicon sequencing on Illumina instruments. In the variant we use most frequently for large-scale projects, we fuse partial adapter sequences (TruSeq or Nextera) ont
Data from: From benchtop to desktop: important considerations when designing amplicon sequencing workflows
负责人:
关键词:
Amplicon Sequencing;high throughput sequencing;DNA metabarcoding;Second generation sequencing;Next-generation sequencing
DOI:
doi:10.5061/dryad.2qf0t
摘要:
Amplicon sequencing has been the method of choice in many high-throughput DNA sequencing (HTS) applications. To date there has been a heavy focus
Data from: A sequel to Sanger: amplicon sequencing that scales
负责人:
关键词:
nucleotide composition;SMRT sequencing;Nuclear DNA;homopolymer;Mitochondrial DNA;DNA Barcoding;phylogenetics;PCR
DOI:
doi:10.5061/dryad.4368h
摘要:
t platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) sequencing implemented
Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
负责人:
关键词:
DNA Barcoding;Genomics\/Proteomics;Next-generation sequencing;insects;species identification;taxonomy;Lepidoptera
DOI:
doi:10.5061/dryad.p25vn
摘要:
, inferior to next-generation sequencers, which are capable of producing millions of sequence reads simultaneously. Additionally, direct Sanger sequencing
Data from: Digital fragment analysis of short tandem repeats by high-throughput amplicon sequencing
负责人:
关键词:
genotyping by sequencing;Ondatra zibethicus;marker;microsatellite;short tandem repeat;length homoplasy;sequencing
DOI:
doi:10.5061/dryad.68mc8
摘要:
-throughput amplicon sequencing approach to fragment analysis of short tandem repeats and characterize its advantages and disadvantages against traditional
Data from: HiMAP: robust phylogenomics from highly multiplexed amplicon sequencing
负责人:
关键词:
multiplex PCR;systematics;Tephritidae;phylogenetics;Amplicon Sequencing;phylogenomics
DOI:
doi:10.5061/dryad.6524f
摘要:
ts using single tube, highly multiplexed amplicon sequencing, which we name HiMAP (Highly Multiplexed Amplicon-based Phylogenomics), and present
Data from: Species tree estimation of North American chorus frogs (Hylidae: Pseudacris) with parallel tagged amplicon sequencing
负责人:
关键词:
reduced representation library;Pseudacris;anonymous nuclear locus;phylogenetics;targeted amplicon sequencing;high-throughput sequencing
DOI:
doi:10.5061/dryad.23rc0
摘要:
for 44 individuals on 1/3 of an Illumina MiSeq run, obtaining 96.5% of the targeted amplicons at less than 20% of the cost of traditional Sanger sequencing
Data from: Genotyping-in-Thousands by sequencing (GT-seq) panel development and application to minimally-invasive DNA samples to support studies
负责人:
关键词:
conservation genetics;Reptiles;Non-invasive sampling;Crotalus oreganus;targeted amplicon sequencing;SNP panel;molecular ecology;RAD-seq;Wildlife Management
DOI:
doi:10.5061/dryad.vr300p0
摘要:
al for harnessing MPS. Here, we test the ability for Genotyping-in-Thousands by sequencing (GT-seq), a multiplex amplicon sequencing approach, to effectively genotype
Data from: High-throughput amplicon sequencing of rRNA genes requires a copy number correction to accurately reflect the effect
负责人:
关键词:
burning;metagenomics;Nitrogen enrichment;Konza prairie;tallgrass prairie;nematodes
DOI:
doi:10.5061/dryad.t8g16
摘要:
of how different nematode species affect, and are affected by, ecosystem processes. High-throughput amplicon sequencing is used to enumerate mic

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