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Data from: Design- and model-based strategies for detecting and quantifying an amphibian pathogen in environmental samples
- 负责人：
- DOI：
- doi:10.5061/dryad.2j0h3
- 摘要：
- on of Batrachochytrium dendrobatidis (Bd) using water filtration. We compared results pre- and post-inhibitor removal, and assessed inferential differences when single versus multiple
Data from: Scrimer: designing primers from transcriptome data
- 负责人：
- DOI：
- doi:10.5061/dryad.2p4t3
- 摘要：
- eline called Scrimer that automates multiple steps, including adaptor removal, read mapping, selection of SNPs and multiple primer design from transcriptome data
Data from: An evaluation of transcriptome-based exon capture for frog phylogenomics across multiple scales of divergence (Class: Amphibia, Order
- 负责人：
- DOI：
- doi:10.5061/dryad.pr3pr
- 摘要：
- to exclude shorter exons (<200 bp). Here, we test directly using transcript sequences for probe design, which are often composed of multiple exons of varying
Data from: Unlocking the story in the swab: A new genotyping assay for the amphibian chytrid fungus Batrachochytrium dendrobatidis
- 负责人：
- DOI：
- doi:10.5061/dryad.33st2
- 摘要：
- well. By leveraging advances in microfluidic multiplex PCR technology and the globally distributed resource of amphibian swab samples, non-invasive skin swabs
Data from: Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model
- 负责人：
- DOI：
- doi:10.5061/dryad.8164
- 摘要：
- equires many PCR replicates, an alternative multiple-step genotyping method is recommended. This model enables researchers to optimize experimental protocol
of multiple nuclear loci
- 负责人：
- DOI：
- doi:10.5061/dryad.fn2nt
- 摘要：
- on, knowledge of parental relationships is essential. One chloroplast and 12 nuclear single-copy loci were amplified by polymerase chain reaction (PCR) in al
Data from: Sequence Capture using PCR-generated Probes (SCPP): a cost-effective method of targeted high-throughput sequencing for non-model organisms
- 负责人：
- DOI：
- doi:10.5061/dryad.rk204
- 摘要：
- cs and phylogenetics of non-model organisms. To multiplex large numbers of indexed samples while sequencing predominantly orthologous, targeted regions of the genome
Data from: De novo discovery and multiplexed amplification of microsatellite markers for black alder (Alnus glutinosa) and related species using SSR
- 负责人：
- DOI：
- doi:10.5061/dryad.gg0jm
- 摘要：
- e tested for amplification, twelve of which were polymorphic in A. glutinosa. These twelve loci were successfully co-amplified in a single multiplex PCR
Data from: Fluidigm2PURC: automated processing and haplotype inference for double-barcoded PCR amplicons
- 负责人：
- DOI：
- doi:10.5061/dryad.89k5k30
- 摘要：
- DNA sequence data for large numbers of individuals at multiple, independent loci. Automated and reproducible processing of these data is a crucial step for researchers
Data from: Environmental metabarcodes for insects: in silico PCR reveals potential for taxonomic bias
- 负责人：
- DOI：
- doi:10.5061/dryad.dn45c
- 摘要：
- Studies of insect assemblages are suited to the simultaneous DNA-based identification of multiple taxa known as metabarcoding. To obtain accurate
