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Data from: Multiplexed microsatellite markers for genetic studies of beech
- 负责人：
- DOI：
- doi:10.5061/dryad.pc8d30j2
- 摘要：
- climate change effects. Multiplex PCR of microsatellites is a fast and cost-effective technique allowing high throughput genotyping. Here we present the procedure use
Data from: Molecular prey identification in Central European piscivores
- 负责人：
- 关键词：
- Diet Analysis Piscivores Predator Prey Interactions Wildlife Management Diagnostic Multiplex PCR
- DOI：
- doi:10.5061/dryad.1vf74
- 摘要：
- sfying using conventional morphological analysis of prey remains. Here, we present a two-step multiplex PCR system, comprised of six assays, allowing for rapid
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
- 负责人：
- DOI：
- doi:10.5061/dryad.591q3
- 摘要：
- to maximize sequencing depth efficiency. The principle benefit of microfluidic PCR over alternative target enrichment strategies is that it bypasses costly
Data from: Modular tagging of amplicons using a single PCR for high-throughput sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.22412
- 摘要：
- to incorporate HTS adapters and MID tags into PCR amplicons are either inefficient, requiring multiple enzymatic reactions and clean-up steps, or costly
Data from: Gene transcription in sea otters (Enhydra lutris): development of a diagnostic tool for sea otter and ecosystem health
- 负责人：
- DOI：
- doi:10.5061/dryad.1nn7v
- 摘要：
- al transcript levels of multiple immune-function genes in the sea otter (Enhydra lutris); sea otter specific, qPCR primer sequences for the genes of interest are defined
Data from: Multiplex preamplification PCR and microsatellite validation allows accurate single nucleotide polymorphism (SNP) genotyping of historical
- 负责人：
- Smith, Matt J.
- 关键词：
- fish scales Fisheries Management Population Genetics - Empirical historical DNA SNP preamplification
- DOI：
- doi:10.5061/dryad.8023
- 摘要：
Data from: Increasing the accuracy and precision of relative telomere length estimates by RT qPCR
- 负责人：
- DOI：
- doi:10.5061/dryad.7356h
- 摘要：
- , indicating storage medium can influence precision. Precision as well as accuracy could be increased by estimating rTL from multiple qPCR reactions and from
Data from: Multiple-strain infections of borrelia afzelii: a role for within-host interactions in the maintenance of antigenic diversity?
- 负责人：
- DOI：
- doi:10.5061/dryad.24k0s
- 摘要：
- esolved the composition of multiple infections using strain-specific PCR assays targeting the ospC gene (which encodes an immunodominant surface protein). We found seve
Data from: Whole transcriptome RNA-Seq analysis of breast cancer recurrence risk using formalin-fixed paraffin-embedded tumor tissue
- 负责人：
- DOI：
- doi:10.5061/dryad.q760h
- 摘要：
- for widely used clinical diagnostic tests; however, RT-PCR is practically constrained in the number of transcripts that can be interrogated. We hav
Data from: Multiplex PCR targeting lineage specific SNPs ? A highly efficient and simple approach to block out predator sequences in molecular
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.44mn451
- 摘要：
- multiplex PCR, considerably reducing the necessary workload. Conclusions: Our multi-locus approach allows the generation of an unprecedented amount of prey
