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Data from: Development of highly reliable in silico SNP resource and genotyping assay from exome capture and sequencing: an example from black spruce
负责人:
关键词:
Environmental DNA;Species Interactions;Bioinfomatics\/Phyloinfomatics;Gymnosperms
DOI:
doi:10.5061/dryad.tr87v
摘要:
ed transcriptome of Picea glauca, a distant relative. A high capture efficiency of 75.9% was reached although spruce has a complex and large genome including gene sequences
Data from: Testing pipelines for genome-wide SNP calling from Genotyping-By-Sequencing (GBS) data for Pinus ponderosa
负责人:
关键词:
pipeline;reference-based;SNP;Pinus ponderosa;de novo;now;GBS
DOI:
doi:10.5061/dryad.6fv8fb4
摘要:
t genomes and their amenability to high-throughput genotyping techniques. Genotyping-by-sequencing (GBS) has been demonstrated to be a robust and cost
Data from: Genotyping-in-Thousands by sequencing (GT-seq) panel development and application to minimally-invasive DNA samples to support studies
负责人:
关键词:
conservation genetics;Reptiles;Non-invasive sampling;Crotalus oreganus;targeted amplicon sequencing;SNP panel;molecular ecology;RAD-seq;Wildlife Management
DOI:
doi:10.5061/dryad.vr300p0
摘要:
for harnessing MPS. Here, we test the ability for Genotyping-in-Thousands by sequencing (GT-seq), a multiplex amplicon sequencing approach, to effectively genotype
Data from: Optimization of the genotyping-by-sequencing strategy for population genomic analysis in conifers
负责人:
关键词:
genotyping-by-sequencing;sequencing depth;SNP validation;library prep parameters;conifer genome;Pinus
DOI:
doi:10.5061/dryad.39gc6
摘要:
Flexibility and low cost make genotyping-by-sequencing (GBS) an ideal tool for population genomic studies of nonmodel species. However, to utilize
Data from: High-throughput microsatellite genotyping in ecology: improved accuracy, efficiency, standardization and success with low-quantity
负责人:
关键词:
faecal DNA;Ursus arctos;individual identification;parentage analysis;high-throughput sequencing;short tandem repeat (STR)
DOI:
doi:10.5061/dryad.18tg7
摘要:
to microsatellite sequences, allowing unambiguous allele identification and enabling automation of the genotyping process through bioinformatics. In addition, the massive
Data from: Large-scale genotyping of highly polymorphic loci by next generation sequencing: how to overcome the challenges to reliably genotype individuals?
负责人:
关键词:
post-processing;parentage relationships;Marmota marmota;high throughput sequencing;non-model species;Holocene;Alpine marmot
DOI:
doi:10.5061/dryad.rp7n9
摘要:
ers of individuals. Next-generation sequencing (NGS) techniques enable such large-scale sequencing, but stringent data processing is required. Here, we develop an easy
Data from: Scrimer: designing primers from transcriptome data
负责人:
关键词:
Luscinia megarhynchos;transcriptome;Luscinia luscinia;454 FLX Titanium
DOI:
doi:10.5061/dryad.2p4t3
摘要:
e is no publicly available software for the automated design of suitable PCR and genotyping primers from next-generation sequence data. Here we present a pi
Data from: Identification and characterization of sex-associated loci in sockeye salmon using genotyping-by-sequencing and comparison with a se
负责人:
关键词:
genotyping-by-sequencing;5\u2019-nuclease assay;Oncorhynchus nerka;sockeye salmon;sex-associated locus;sdY;Holocene;Sex Identification
DOI:
doi:10.5061/dryad.p15br
摘要:
for associations between sex and genotypes at thousands of loci available from a genotyping-by-sequencing (GBS) dataset to discover sex-associated loci
Data from: Extending RAD tag analysis to microbial ecology: a comparison between multi locus sequence typing (MLST) and 2b-RAD to investigate
负责人:
关键词:
Genomics\/Proteomics;Listeria monocytogenes;bacteria;Ecological Genetics;Population Genetics - Empirical
DOI:
doi:10.5061/dryad.m1bm5
摘要:
sequencing approaches, however, such as MultiLocus Sequence Typing (mlst) are quite laborious and time-consuming, especially for large-scale analyse
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
负责人:
关键词:
Genomics\/Proteomics;Natural Selection and Contemporary Evolution;phylogeography;molecular evolution;quantitative genetics;Ecological Genetics
DOI:
doi:10.5061/dryad.7tb72
摘要:
associated DNA sequencing (RAD sequencing or RADseq) has become an established method for SNP genotyping on Illumina sequencing platforms. Her

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