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Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility
负责人:
Porter, Teresita
关键词:
ancient DNA contamination reproducibility barcoding Beringia amplicon pyrosequencing
DOI:
doi:10.5061/dryad.4m7d1
摘要:
DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we asse
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
负责人:
关键词:
Illumina MiSeq;microfluidic PCR;genome-tagged amplification;Miocene;Burseraceae;targeted amplicon sequencing;Commiphora;phylogenomics;Bursera
DOI:
doi:10.5061/dryad.591q3
摘要:
angiosperm taxa from these publicly available datasets; 2) that microfluidic PCR amplification followed by high throughput sequencing can produce highly
Data from: Targeted gene enrichment and high-throughput sequencing for environmental biomonitoring: a case study using freshwater macroinvertebrates
负责人:
关键词:
Amplicon Sequencing;biomonitoring;Gene Enrichment;macroinvertebrates;Holocene
DOI:
doi:10.5061/dryad.67n7n
摘要:
using the gene-enrichment method (all genes). Our data indicated primer biases occurred during amplicon metabarcoding with greater than 80% of sequence
Data from: Unraveling independent origins of two tetraploid Achillea species by amplicon sequencing
负责人:
关键词:
Achillea;Allopolyploid speciation;?amplicon?sequencing;population genetic structure;demographic history
DOI:
doi:10.5061/dryad.v652k70
摘要:
t allopolyploidization between the same two parental species may lead to the origin of different taxa has received little attention. Here we used a new progenitor-specific amplicon sequencing
Data from: Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA
负责人:
关键词:
mitogenome sequencing;Actinopterygii;eDNA
DOI:
doi:10.5061/dryad.q5gg0
摘要:
of fish species assembled from pooling genomic DNA extracted from tissues. In situ we utilized long-range PCR and Illumina sequencing to generate fragments bet
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
负责人:
关键词:
moss;diversity;Peltigera praetextata;Brachythecium fendleri;454 amplicon pyrosequencing;ITSrDNA;lichen;Pinus leiophylla var. chihuahuana;mycobiont
DOI:
doi:10.5061/dryad.8271f
摘要:
d in 454 amplicon pyrosequencing might influence ecological inferences in studies of endophytic and endolichenic fungi. We first contrast 454 data se
Data from: Comparison of 454 pyrosequencing methods for characterizing the major histocompatibility complex of nonmodel species and the advantages
负责人:
关键词:
454 sequencing;wolverine;Gulo gulo;multi-locus genes;pyrosequencing;Major histocompatibility complex;Holocene
DOI:
doi:10.5061/dryad.s5b40
摘要:
in population genetic studies. We compared two sample tagging protocols and two bioinformatic procedures for 454 sequencing through characterization of a 185-bp fragment
Data from: Within-host competition between Borrelia afzelii ospC strains in wild hosts as revealed by massively parallel amplicon sequencing
负责人:
关键词:
mixed infections;ospC;Virulence;Borrelia afzelii;within-host competition
DOI:
doi:10.5061/dryad.vf83f
摘要:
a protocol for 454 amplicon sequencing of ospC, which allows both detection and quantification of each individual strain in an infection. Each host individual
Data from: Efficient detection of novel nuclear markers for Brassicaceae by transcriptome sequencing
负责人:
关键词:
Primer-design;Arabidopsis thaliana;primer database;Next-generation sequencing;RNA-seq;phylogenetics;Cardamine hirsuta;Arabis alpina;454 transcriptome sequencing;Brassicaceae
DOI:
doi:10.5061/dryad.63j2j
摘要:
transcriptome sequencing reads from two of the distantly related Brassicaceae species, Cardamine hirsuta and Arabis alpina, onto the Arabidopsis thaliana reference genome

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