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Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
- 负责人:
- DOI:
- doi:10.5061/dryad.v7f7r
- 摘要:
- whether the estimated frequency of representative sequences, generated by 454 amplicon sequencing, differs significantly from that obtaine
Data from: Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)
- 负责人:
- 关键词:
- DOI:
- doi:10.5061/dryad.n8v4v6d
- 摘要:
- for amplicon sequencing on Illumina instruments. In the variant we use most frequently for large-scale projects, we fuse partial adapter sequences (TruSeq or Nextera) ont
Data from: From benchtop to desktop: important considerations when designing amplicon sequencing workflows
- 负责人:
- DOI:
- doi:10.5061/dryad.2qf0t
- 摘要:
- Amplicon sequencing has been the method of choice in many high-throughput DNA sequencing (HTS) applications. To date there has been a heavy focus
Data from: A sequel to Sanger: amplicon sequencing that scales
- 负责人:
- DOI:
- doi:10.5061/dryad.4368h
- 摘要:
- t platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) sequencing implemented
Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
- 负责人:
- DOI:
- doi:10.5061/dryad.p25vn
- 摘要:
- , inferior to next-generation sequencers, which are capable of producing millions of sequence reads simultaneously. Additionally, direct Sanger sequencing
Data from: Digital fragment analysis of short tandem repeats by high-throughput amplicon sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.68mc8
- 摘要:
- -throughput amplicon sequencing approach to fragment analysis of short tandem repeats and characterize its advantages and disadvantages against traditional
Data from: HiMAP: robust phylogenomics from highly multiplexed amplicon sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.6524f
- 摘要:
- ts using single tube, highly multiplexed amplicon sequencing, which we name HiMAP (Highly Multiplexed Amplicon-based Phylogenomics), and present
Data from: Species tree estimation of North American chorus frogs (Hylidae: Pseudacris) with parallel tagged amplicon sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.23rc0
- 摘要:
- for 44 individuals on 1/3 of an Illumina MiSeq run, obtaining 96.5% of the targeted amplicons at less than 20% of the cost of traditional Sanger sequencing
Data from: Genotyping-in-Thousands by sequencing (GT-seq) panel development and application to minimally-invasive DNA samples to support studies
- 负责人:
- DOI:
- doi:10.5061/dryad.vr300p0
- 摘要:
- al for harnessing MPS. Here, we test the ability for Genotyping-in-Thousands by sequencing (GT-seq), a multiplex amplicon sequencing approach, to effectively genotype
Data from: High-throughput amplicon sequencing of rRNA genes requires a copy number correction to accurately reflect the effect
- 负责人:
- DOI:
- doi:10.5061/dryad.t8g16
- 摘要:
- of how different nematode species affect, and are affected by, ecosystem processes. High-throughput amplicon sequencing is used to enumerate mic