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Data from: Metabarcoding and mitochondrial metagenomics of endogean arthropods to unveil the mesofauna of the soil
- 负责人:
- DOI:
- doi:10.5061/dryad.5t5s1
- 摘要:
- of arthropod communities from these bulk DNA extractions using cox1 metabarcoding and shotgun metagenomic sequencing on the MiSeq Illumina platform. The FBF protocol
Data from: Comparison and validation of Oomycetes metabarcoding primers for Phytophthora high throughput sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.68tk49g
- 摘要:
- . The three nested PCR strategies proved to be a reliable qualitative approach, identifying approximately 95% of the species after Illumina Miseq se
Data from: Environmental filtering by pH and soil nutrients drives community assembly in fungi at fine spatial scales
- 负责人:
- DOI:
- doi:10.5061/dryad.q0rv9
- 摘要:
- and edaphic environment on associated fungal communities. We identified fungal community composition with Illumina sequencing of ITS amplicons, measured al
Data from: Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)
- 负责人:
- DOI:
- doi:10.5061/dryad.mf1ck
- 摘要:
- in quality from nearly intact to highly sheared. All samples were sequenced as a multiplexed pool on an Illumina MiSeq. Libraries created from low
Data from: Isolation, characterization, and cross-amplification of 20 microsatellite markers for the rare Conospermum undulatum (Proteaceae)
- 负责人:
- DOI:
- doi:10.5061/dryad.f81k3q7
- 摘要:
- rvation purposes and to facilitate ecological studies. Methods and Results: Illumina MiSeq high-throughput sequencing was used to develop 20 nove
Data from: Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients
- 负责人:
- DOI:
- doi:10.5061/dryad.5gc82
- 摘要:
- -end sequencing. Illumina Miseq System was used for sequencing, and Quantitative Insights Into Microbial Ecology (QIIME) and Stata were used for bioinformatics
Data from: Isolation of microsatellite loci by next-generation sequencing of the critically endangered Northern Bald ibis, Geronticus eremita
- 负责人:
- DOI:
- doi:10.5061/dryad.h1167
- 摘要:
- microsatellite markers, specific for the Northern Bald Ibis, Geronticus eremita (LINNAEUS, 1785), have been isolated from next-generation sequencing (Illumina MiSeq
Data from: Family-assisted inference of the genetic architecture of MHC variation
- 负责人:
- DOI:
- doi:10.5061/dryad.rm611
- 摘要:
- ), including 590 offspring from 130 families using Illumina MiSeq technology. Coupled with a high per-individual sequencing coverage (~3000×), the study
Data from: A broadly applicable COI primer pair and an efficient single?tube amplicon library preparation protocol for metabarcoding
- 负责人:
- 关键词:
- DOI:
- doi:10.5061/dryad.5jq5t36
- 摘要:
- the target gene, and attach MIDs and Illumina sequencing adapters in one reaction. This eliminates the need for re-amplification or enzymatic ligation duri
Data from: Soil organic matter quantity and quality shape microbial community compositions of subtropical broadleaved forests
- 负责人:
- DOI:
- doi:10.5061/dryad.8071s
- 摘要:
- e is known about their belowground ecosystems despite their ecological importance in driving biogeochemical cycling. Here, we used Illumina MiSeq sequencing targeting