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共检索到32条 ,权限内显示50条;
Data from: Species tree estimation of North American chorus frogs (Hylidae: Pseudacris) with parallel tagged amplicon sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.23rc0
- 摘要：
- for 44 individuals on 1/3 of an Illumina MiSeq run, obtaining 96.5% of the targeted amplicons at less than 20% of the cost of traditional Sanger se
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
- 负责人：
- DOI：
- doi:10.5061/dryad.591q3
- 摘要：
- and Illumina MiSeq, we generated nuclear, subgenomic libraries for 96 species simultaneously and sequenced them for a total cost of ca. $6000 USD. Approximately half
Data from: A from-benchtop-to-desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies
- 负责人：
- DOI：
- doi:10.5061/dryad.f40v5
- 摘要：
- was amplified by combining two minibarcoding primer sets to maximize the taxonomic coverage. Amplicons were sequenced by an Illumina MiSeq platform. We developed
Data from: The effects of read length, quality and quantity on microsatellite discovery and primer development: from Illumina to PacBio
- 负责人：
- DOI：
- doi:10.5061/dryad.r20t0
- 摘要：
- , while platforms such as Illumina MiSeq will provide cost-efficient solutions for multi-species microsatellite projects.
Data from: Disease-free monoculture farming by fungus-growing termites
- 负责人：
- DOI：
- doi:10.5061/dryad.t6t12
- 摘要：
- termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi
Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community
- 负责人：
- DOI：
- doi:10.5061/dryad.tn6vn
- 摘要：
- community-wide sequencing effort. Here we use Fluidigm’s micro-fluidic Access Arrays (IFC) followed by Illumina? MiSeq Nano sequencing on a culture-derived
Data from: 2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador
- 负责人：
- DOI：
- doi:10.5061/dryad.02bf1
- 摘要：
- collected from the central coast and southern Andean region of Ecuador, from June 2006 to July 2013. 2b-RAD sequencing data was performed on an Illumina MiSeq
Data from: Analysis of microsatellite loci in tree of heaven (Ailanthus altissima (Mill.) Swingle) using SSR-GBS
- 负责人：
- 关键词：
- genotyping by sequencing;SNP;microsatellite;Amplicon Sequencing;Ailanthus altissima;Tree of heaven
- DOI：
- doi:10.5061/dryad.873b2j2
- 摘要：
- w microsatellite markers for the tree of heaven by applying an NGS-based method using the Illumina MiSeq platform. NGS technology was proved to be an effective method
Data from: Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska
- 负责人：
- DOI：
- doi:10.5061/dryad.p1602
- 摘要：
- This study investigated soil bacterial and archaeal communities by Illumina MiSeq sequencing of 16S rRNA gene amplicons across a permafrost thaw gradient
Data from: Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype calling accuracy
- 负责人：
- DOI：
- doi:10.5061/dryad.34dt1
- 摘要：
- four-base tag to the first index read, which allows duplicate discrimination in aligned data. This approach was validated on the Illumina MiSeq platform, usi
