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Data from: Fluorescent protein-mediated colour polymorphism in reef corals: multi-copy genes extend the adaptation/acclimatization potential to variable light environments
- 负责人：
- DOI：
- doi:10.5061/dryad.5d079
- 摘要：
- . We have explored the striking intraspecific variability in the expression of coral pigments from the green fluorescent protein (GFP) family to elucidate
Raw data.Immunohistochemistry, RNA expression and lab. values per experimental animal.
- 负责人：
- 关键词：
- Tissue Engineering Inflammation Bone Marrow Transplantation Vascular Graft Rat Model Chronic Kidney Disease Vascular Access
- DOI：
- doi:10.17632/gs5632dzbc.1
- 摘要：
- , we utilized a rat model of CKD, which we combined with a BM-transplantation using GFP-labeled cells. These experiments revealed that both bone-marrow derived
Data from: Quantifying episodes of sexual selection: insights from a transparent worm with fluorescent sperm
- 负责人：
- DOI：
- doi:10.5061/dryad.p093c
- 摘要：
- d a transgenic line of the transparent flatworm, Macrostomum lignano, which expresses green fluorescent protein (GFP) in all cell types, including sperm cells
Correlative Light and Electron Microscopy for the Investigation of Muscle Disease
- 负责人：
- 关键词：
- Genetics
- DOI：
- doi:10.4225/03/55babdb6578a9
- 摘要：
- , we created a zebrafish model. To generate the model we expressed a disease causing form of ACTA1 (ACTA1D286G) tagged with enhanced green fluorescent protein
Data from: Screening of cytotoxic or cytostatic flavonoids with quantitative FUCCI-based cell cycle assay
- 负责人：
- DOI：
- doi:10.5061/dryad.40cd5ps
- 摘要：
- the cell cycle in individual live cells over time by determining the ratios between red fluorescence (RF) of RFP-Cdt1 and green fluorescence (GF) of GFP-Geminin
Data from: Calcium imaging with genetically encoded sensor Case12: facile analysis of ?7/?9 nAChR mutants
- 负责人：
- DOI：
- doi:10.5061/dryad.4k831
- 摘要：
- calcium ion sensor followed by analysis of their pharmacology using a fluorescence microscope or a fluorometric imaging plate reader (FLIPR) with a GFP filter
Data from: Differential responses of coral larvae to the colour of ambient light guide them to suitable settlement microhabitat
- 负责人：
- DOI：
- doi:10.5061/dryad.1c4m9
- 摘要：
- Reef-building corals produce planktonic planula larvae that must select an appropriate habitat to settle and spend the rest of their life, a behaviour that plays a critical role in survival. Here, we report that larvae obtained from a deep-water population of Pseudodiploria strigosa settled more readily under blue light and in the dark, which aligns well with the light field characteristics of their natal habitat. By contrast, larvae of the shallow-water coral Acropora millepora settled in high proportions under blue and green light while settlement was less in the dark. Acropora millepora larvae also showed reduced settlement under red light, which should be abundant at shallow depth. Hypothesizing that this might be a mechanism preventing the larvae from settling on the exposed upwards-facing surfaces, we quantified A. millepora settlement in manipulated light chambers in situ on the reef. While A. millepora larvae naturally preferred settling on vertical rather than exposed horizontal surfaces, swapping the colours of upwards-facing and sideways-facing light fields was sufficient to invert this preference. We also tested if the variation in intrinsic red fluorescence in A. millepora larvae correlates with settlement rates, as has been suggested previously. We observed this correlation only in the absence of light, indicating that larval red fluorescent protein is probably not directly involved in light sensing. Our study reveals previously under-appreciated light-sensory capabilities in coral larvae, which could be an important axis of ecological differentiation between coral species and/or populations.
Data from: Homeostasis of metabolites in Escherichia coli on transition from anaerobic to aerobic conditions and the transient secretion of pyruvate
- 负责人：
- DOI：
- doi:10.5061/dryad.1n542
- 摘要：
- -green fluorescent protein or PykF-FLAG fusion proteins. Measurements showed that PykF-FLAG relocates to the cell membrane within 5?min of aeration and then slowly returns
Data from: Threshold effect of growth rate on population variability of Escherichia coli cell lengths
- 负责人：
- DOI：
- doi:10.5061/dryad.2bs69
- 摘要：
- t RecA-GFP protein recruitment to nucleoids is more frequent at growth rates exceeding the growth rate threshold and is further enhanced on HU treatment
Data from: NF-?B oscillations translate into functionally related patterns of gene expression
- 负责人：
- DOI：
- doi:10.5061/dryad.j62n1
- 摘要：
- t can be entrained, that is, reach a persistent synchronized state through small periodic perturbations. We show here that for our GFP-p65 knock-in cells NF-?B behaves
