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Data from: Fluorescent sperm in a transparent worm: validation of a GFP marker to study sexual selection
- 负责人：
- DOI：
- doi:10.5061/dryad.tq43g
- 摘要：
- . In the transparent free-living flatworm Macrostomum lignano, a recently established transgenic line that expresses green fluorescent protein (GFP) in all cell types
Data from: Acclimatization of symbiotic corals to mesophotic light environments through wavelength transformation by fluorescent protein pigments
- 负责人：
- DOI：
- doi:10.5061/dryad.19sq4
- 摘要：
- intensities in shallow water, the spectral transformation observed in deeper-water corals highlights the importance of GFP-like protein expression as an ecological mechanism
Data from: PCR duplication: a one-step cloning-free method to generate duplicated chromosomal loci and interference-free expression reporters in yeast
- 负责人：
- DOI：
- doi:10.5061/dryad.gj51n
- 摘要：
- inserted a GFP downstream of it. This created a reporter for promoter activity while leaving the FAR1 gene fully intact. In another experiment, we used PCR
Data from: Manipulation of the N-terminal sequence of the Borna disease virus X protein improves its mitochondrial targeting and neuroprotective potential
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.1rs12
- 摘要：
- rent domains of X fused to enhanced green fluorescent protein (eGFP) and compared their subcellular localization to that of eGFP. We observed tha
Data from: Live imaging of symbiosis: spatiotemporal infection dynamics of GFP-labelled Burkholderia symbiont in the bean bug Riptortus pedestris
- 负责人：
- DOI：
- doi:10.5061/dryad.q1r19
- 摘要：
- with green fluorescent protein (GFP), which enabled detailed observation of spatiotemporal dynamics and the colonization process of the symbiont in freshly prepared specimens
Data from: In vivo tracking of dendritic cell using MRI reporter gene, ferritin
- 负责人：
- DOI：
- doi:10.5061/dryad.d08h6
- 摘要：
- immunotherapeutic strategies. This study is to investigate the influence of transduction of human ferritin heavy chain (FTH) and green fluorescence protein (GFP
Data from: Sex allocation adjustment to mating group size in a simultaneous hermaphrodite
- 负责人：
- DOI：
- doi:10.5061/dryad.16vk4
- 摘要：
- with ubiquitous expression of green-fluorescent protein (GFP), we assessed the number of wild-type mating partners carrying GFP-positive sperm from these focal
Data from: Determining the subcellular location of new proteins from microscope images using local features
- 负责人：
- DOI：
- doi:10.5061/dryad.2vm70
- 摘要：
- Motivation: Evaluation of previous systems for automated determination of subcellular location from microscope images has been done using datasets in which each location class consisted of multiple images of the same representative protein. Here, we frame a more challenging and useful problem where previously unseen proteins are to be classified. Results: Using CD-tagging, we generated two new image datasets for evaluation of this problem, which contain several different proteins for each location class. Evaluation of previous methods on these new datasets showed that it is much harder to train a classifier that generalizes across different proteins than one that simply recognizes a protein it was trained on. We therefore developed and evaluated additional approaches, incorporating novel modifications of local features techniques. These extended the notion of local features to exploit both the protein image and any reference markers that were imaged in parallel. With these, we obtained a large accuracy improvement in our new datasets over existing methods. Additionally, these features help achieve classification improvements for other previously studied datasets.
Data from: Covariance among premating, postcopulatory and viability fitness components in Drosophila melanogaster and their influence on paternity measurement
- 负责人：
- DOI：
- doi:10.5061/dryad.467r0
- 摘要：
- green fluorescent protein (i.e., GFP) in a series of competitive and non-competitive matings. This approach permitted us to track paternity-specific viability over al
Data from: Caenorhabditis elegans genes affecting interindividual variation in life-span biomarker gene expression
- 负责人：
- DOI：
- doi:10.5061/dryad.m55h6
- 摘要：
- Caenorhabditis elegans, differences in Green Fluorescent Protein (GFP) expressed from the hsp-16.2 promoter predict differences in life span. Thus, it is of int
