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Data from: Mitochondrial capture enriches mito-DNA 100 folds enabling PCR-free mitogenomics biodiversity analysis
- 负责人:
- DOI:
- doi:10.5061/dryad.19nf6
- 摘要:
- impediment of such a method is the lack of appropriate mitochondrial DNA enrichment ways. Because mitochondrial genomes (mitogenomes) make up only a sma
Data from: Exon capture phylogenomics: efficacy across scales of divergence
- 负责人:
- DOI:
- doi:10.5061/dryad.34274
- 摘要:
- e identified in the transcriptomes of selected probe design (PD) samples. The target exons are then enriched in sample DNA libraries prior to performing HTS
Data from: Genomic sequence capture of haemosporidian parasites: methods and prospects for enhanced study of host-parasite evolution
- 负责人:
- DOI:
- doi:10.5061/dryad.8030nq9
- 摘要:
- ted. Phylogenetic relationships estimated with >300 nuclear loci were well resolved, providing substantial improvement over the mtDNA barcode. We provide pro
Data from: Recognizing false positives: synthetic oligonucleotide controls for environmental DNA surveillance
- 负责人:
- DOI:
- doi:10.5061/dryad.h373j
- 摘要:
- sequencing. For quantitative PCR (qPCR), false positives in environmental samples can also be detected using a fluorescent probe designed to detect the synthetic insert. The gene
Data from: High phylogenetic utility of an ultraconserved element probe set designed for Arachnida
- 负责人:
- Derkarabetian, Shahan
- DOI:
- doi:10.5061/dryad.5k3j3
- 摘要:
- , have hindered progress. Here, we present a genomic toolkit for arachnids featuring hundreds of conserved DNA regions (ultraconserved elements or UCEs
Procambarus clarkii in freshwater ponds
- 负责人:
- DOI:
- doi:10.5061/dryad.0ck3q
- 摘要:
- , the red swamp crayfish Procambarus clarkii. Species-specific primers and probes were designed; their specificity was tested using in silico PCR simulations and ag
Data from: Cost-efficient high throughput capture of museum arthropod specimen DNA using PCR-generated baits
- 负责人:
- DOI:
- doi:10.5061/dryad.8004512
- 摘要:
- h as arthropods. Next generation sequencing techniques allow for sequencing of short DNA fragments contained in preserved specimens >20 years old, but approaches
Data from: Development of highly reliable in silico SNP resource and genotyping assay from exome capture and sequencing: an example from black spr
- 负责人:
- DOI:
- doi:10.5061/dryad.tr87v
- 摘要:
- and genomic selection approaches are being developed. Targeted sequencing was achieved after capturing P. mariana exome with probes designed from the sequence
Data from: Oral microflora in preschool children attending a fluoride varnish program: a cross-sectional study
- 负责人:
- 关键词:
- preschool children;community health program;bacteria;fluoride varnish;caries prevention;2013-2014
- DOI:
- doi:10.5061/dryad.7v387
- 摘要:
- DNA-DNA hybridization using 12 pre-determined bacterial probes. Caries and background data were collected from clinical examinations and questionnaires. Results: Gram
Data from: Environmental DNA surveys help to identify winter hibernacula of a temperate freshwater turtle
- 负责人:
- DOI:
- doi:10.5061/dryad.3j9kd51d1
- 摘要:
- with a species?specific probe to quantify concentrations of map turtle eDNA.
Results and discussion
We found localized eDNA signals consistent