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Data from: More than skin and bones: comparing extraction methods and alternative sources of DNA from avian museum specimens
负责人:
关键词:
toe pad;historical DNA;DNA extraction;avian museum specimens;skin punch;phenol-chloroform;natural history collections
DOI:
doi:10.5061/dryad.0nj71g0
摘要:
can be a poor source of DNA. In this study, we apply two DNA extraction methods (phenol-chloroform and silica column) to three different sources of DNA (toe pad
Data from: Topological DNA-binding of SMC-like RecN promotes RecA-mediated DNA double-strand break repair
负责人:
关键词:
DNA homologous recombination repair;DNA double strand break;Escherichia coli
DOI:
doi:10.5061/dryad.7p3p8j3
摘要:
of DNA double-strand breaks (DSBs) by homologous recombination. However, the understanding of how RecN acts in concert with the RecA recombinase to promote DSB repair remains
Data from: Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae
负责人:
关键词:
Environmental DNA;Chironomidae;DNA Barcoding;insects;species identification;Community Ecology;Invertebrates;COI;Ecological Genetics;Aquatic Macroinvertebrates
DOI:
doi:10.5061/dryad.tf6r4
摘要:
e, we compare the relative effectiveness of five different DNA extraction protocols and direct PCR in isolation of DNA from chironomid pupal exuviae. Chironomidae
Data from: Evaluating DNA degradation rates in faecal pellets of the endangered pygmy rabbit
负责人:
关键词:
Brachylagus idahoensis;pygmy rabbit;genotyping error;noninvasive genetic sampling;DNA degradation
DOI:
doi:10.5061/dryad.722qb
摘要:
(Brachylagus idahoensis). To investigate this method’s efficiency for pygmy rabbit monitoring, we evaluated the effect of sample age on DNA degradation in faecal pellets
Data from: Protocols for dry DNA storage and shipment at room temperature
负责人:
关键词:
DNA preservation;Biomatrica;trehalose;PVA;dry storage
DOI:
doi:10.5061/dryad.6p2h0
摘要:
The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment
Data from: Clearing muddied waters: capture of environmental DNA from turbid waters
负责人:
关键词:
terrestrial;Environmental DNA;turbid;optimization
DOI:
doi:10.5061/dryad.835s6
摘要:
Understanding the differences in efficiencies of various methods to concentrate, extract, and amplify environmental DNA (eDNA) is vital for best
Data from: Factors influencing detection of eDNA from a stream-dwelling amphibian
负责人:
关键词:
Amphibians Environmental DNA Idaho giant salamander DNA degradation qPCR
DOI:
doi:10.5061/dryad.mt60p
摘要:
Environmental DNA (eDNA) methods for detecting and estimating abundance of aquatic species are emerging rapidly, but littl
Data from: Development and validation of rapid environmental DNA (eDNA) detection methods for bog turtle (Glyptemys muhlenbergii)
负责人:
关键词:
bog turtle;C. elegans;DNA extraction;Environmental DNA;internal control;Glyptemys muhlenbergii;sample interference;qPCR inhibition;eDNA;DNA recovery
DOI:
doi:10.5061/dryad.6n7675r
摘要:
insculpta ). Additionally, substrates containing bog turtle eDNA are often rich in organics or other substances that frequently inhibit both DNA extraction
Data from: The release rate of environmental DNA from juvenile and adult fish
负责人:
关键词:
Lepomis macrochirus;Environmental DNA;quantitative PCR;Centrarchidae
DOI:
doi:10.5061/dryad.3qb7t
摘要:
The environmental DNA (eDNA) technique is expected to become a powerful, non-invasive tool for estimating the distribution and biomass of organisms
Data from: Behavioral vs. molecular sources of conflict between nuclear and mitochondrial DNA: the role of male-biased dispersal in a Holarctic
负责人:
关键词:
gene flow;Mergus;Mergus merganser;monophyletic;Multi-locus;Holocene;coalescent;simulations;Pleistocene
DOI:
doi:10.5061/dryad.vc8ch8r8
摘要:
Genetic studies of waterfowl (Anatidae) have observed the full spectrum of mitochondrial (mt) DNA population divergence, from panmixia to deep

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