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Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人：
- DOI：
- doi:10.5061/dryad.28n8d6t
- 摘要：
- targeting the mRNA sequence extending beyond the dsRNA target region, regardless of the expression levels of the target gene. Our results suggest tha
Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
- 负责人：
- DOI：
- doi:10.5061/dryad.179116t
- 摘要：
- ee approaches, detecting 50% of the annotated genomic ORs, and targeted sequence capture recovered nearly 75% of annotated genes. Each sequencing approach
Data from: Phylogenomics from whole genome sequences using aTRAM
- 负责人：
- DOI：
- doi:10.5061/dryad.26j38
- 摘要：
- of automated Target Restricted Assembly Method (aTRAM) to assemble 1107 single-copy ortholog genes from whole genome sequencing of sucking lice (Anoplura
Data from: Efficiency of ddRAD target enriched sequencing across spiny rock lobster species (Palinuridae: Jasus)
- 负责人：
- DOI：
- doi:10.5061/dryad.3dk40
- 摘要：
- Double digest restriction site-associated DNA sequencing (ddRADseq) and target capture sequencing methods are used to explore population and phyloge
Data from: Targeted sequence capture and resequencing implies a predominant role of regulatory regions in the divergence of a sympatric
- 负责人：
- DOI：
- doi:10.5061/dryad.3nc54
- 摘要：
- ally in the case of recently duplicated genomes as in salmonids, as this greatly complicates whole genome resequencing. We thus designed a sequence capture ar
Data from: Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae
- 负责人：
- DOI：
- doi:10.5061/dryad.r9c12
- 摘要：
- application, targeted enrichment simplifies the informatics challenge of identifying orthologous loci associated with de novo genome sequencing. We conclude tha
Data from: Exon capture phylogenomics: efficacy across scales of divergence
- 负责人：
- DOI：
- doi:10.5061/dryad.34274
- 摘要：
- . High Throughput Sequencing (HTS) is now providing opportunities to incorporate thousands of loci in ‘phylogenomic’ studies. Here, we used target enrichment to sequence c.3000
Data from: A targeted next-generation sequencing toolkit for exon-based cichlid phylogenomics
- 负责人：
- DOI：
- doi:10.5061/dryad.298qm
- 摘要：
- chlid genomes and sequence the targeted exons for a range of divergent (> tens of millions of years) taxa with probes designed from a single reference speci
Data from: Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: compar
- 负责人：
- DOI：
- doi:10.5061/dryad.3447g
- 摘要：
- Objective: To validate the targeted next-generation sequencing (NGS) platform-Ion Torrent PGM for KRAS exon 2 and expanded RAS mutations dete
Data from: A phylogenomic approach based on PCR target enrichment and high throughput sequencing: resolving the diversity within the South American
- 负责人：
- DOI：
- doi:10.5061/dryad.fh592
- 摘要：
- Advances in high-throughput sequencing (HTS) have allowed researchers to obtain large amounts of biological sequence information at speeds and costs
