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Data from: Lessons learned from microsatellite development for non-model organisms using 454 pyrosequencing
负责人:
关键词:
Comparative studies;shotgun sequencing;next generation sequencing technology;population genetics;massively parallel sequencing;conservation genetics
DOI:
doi:10.5061/dryad.22p7m
摘要:
. Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencing
Data from: Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)
负责人:
关键词:
Acipenser fulvescens;polyploidy;Trichomonas;transcriptome;Pyrosequencing 454;Schistosoma
DOI:
doi:10.5061/dryad.q0740kp8
摘要:
Modern pyrosequencing has the potential to uncover many interesting aspects of genome evolution, even in lineages where genomic resources are scarce
Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms
负责人:
关键词:
DNA Barcoding;metagenomics;Bacillariophyta;diatoms;Algae;Community Ecology
DOI:
doi:10.5061/dryad.370nq
摘要:
environmental communities. In this framework, we tested the possibility to derive accurate inventories of diatom communities from pyrosequencing outputs
Data from: Cross-species transferability of SSR loci developed from transcriptome sequencing in lodgepole pine
负责人:
关键词:
454;SSR;Pinus ponderosa;EST;Pinus contorta
DOI:
doi:10.5061/dryad.8dk6t78s
摘要:
ulting from 454 pyrosequencing of Pinus contorta cDNA were identified. From these we developed PCR primers for approximately 4000 candidate SSRs. Her
Data from: A next-generation sequencing approach to river biomonitoring using benthic diatoms
负责人:
关键词:
biomonitoring;Bacillariophyta;molecular ecology;diatoms;benthic diatom communities;species inventories;Next-generation sequencing
DOI:
doi:10.5061/dryad.3gm8c
摘要:
. Next Generation Sequencing (NGS) can be used to study communities of microorganisms, so we carried out a test of the reliability of 454 pyrosequencing for estimating diatom
Data from: Comparison of 454 pyrosequencing methods for characterizing the major histocompatibility complex of nonmodel species and the advantages
负责人:
关键词:
454 sequencing;wolverine;Gulo gulo;multi-locus genes;pyrosequencing;Major histocompatibility complex;Holocene
DOI:
doi:10.5061/dryad.s5b40
摘要:
s of assessment often underestimate total variation. Here, we explored the suitability of 454 pyrosequencing for characterizing multilocus genes for use
-enriched shotgun pyrosequencing.
负责人:
关键词:
high throughput genotyping;Next-generation sequencing;short sequence repeats;Alnus incana;Alnus cordata;common alder;Alnus glutinosa;454 GS-FLX Titanium
DOI:
doi:10.5061/dryad.gg0jm
摘要:
ive high quality microsatellite marker discovery in non-model organisms for which no genomic information is available. Here, we use shotgun pyrosequencing
Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
负责人:
关键词:
DNA Barcoding;Genomics\/Proteomics;Next-generation sequencing;insects;species identification;taxonomy;Lepidoptera
DOI:
doi:10.5061/dryad.p25vn
摘要:
to DNA barcoding PCR primers. We employ 454 pyrosequencing to recover full-length DNA barcodes of 190 specimens using 12.5% capacity of a 454 sequencing ru
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility
负责人:
Porter, Teresita
关键词:
ancient DNA contamination reproducibility barcoding Beringia amplicon pyrosequencing
DOI:
doi:10.5061/dryad.4m7d1
摘要:
DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we asse
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
负责人:
关键词:
moss;diversity;Peltigera praetextata;Brachythecium fendleri;454 amplicon pyrosequencing;ITSrDNA;lichen;Pinus leiophylla var. chihuahuana;mycobiont
DOI:
doi:10.5061/dryad.8271f
摘要:
d in 454 amplicon pyrosequencing might influence ecological inferences in studies of endophytic and endolichenic fungi. We first contrast 454 data se

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