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Data from: Substantial differences in bias between single-digest and double-digest RAD-seq libraries: a case study
负责人:
关键词:
selection components analysis;Syngnathus scovelli;Next-generation sequencing;allele dropout;PCR duplicates
DOI:
doi:10.5061/dryad.qf916
摘要:
The trade?offs of using single?digest vs. double?digest restriction site?associated DNA sequencing (RAD?seq) protocols have been widely discusse
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
负责人:
关键词:
Genomics\/Proteomics;Natural Selection and Contemporary Evolution;phylogeography;molecular evolution;quantitative genetics;Ecological Genetics
DOI:
doi:10.5061/dryad.7tb72
摘要:
e, we developed a protocol and adapters for double-digest RAD sequencing for Ion Torrent (Life Technologies; Ion Proton, Ion PGM) semiconductor sequencing
Data from: Efficiency of ddRAD target enriched sequencing across spiny rock lobster species (Palinuridae: Jasus)
负责人:
关键词:
Jasus edwardsii;variant-calling file;probe sequences;Sagmariasus verreauxi;probes sequences
DOI:
doi:10.5061/dryad.3dk40
摘要:
Double digest restriction site-associated DNA sequencing (ddRADseq) and target capture sequencing methods are used to explore population and phyloge
Data from: Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype calling accuracy
负责人:
关键词:
Wasmannia auropunctata;Mixornis gularis;Next-generation sequencing;genotyping;Saccharomyces cervisiae;RAD-seq;methodology
DOI:
doi:10.5061/dryad.34dt1
摘要:
ng it difficult to estimate the extent of PCR duplication. Here, we introduce a slight change to the Illumina sequencing adaptor chemistry, appending a unique
Data from: TERAD: Extraction of transposable element composition from RADseq data
负责人:
关键词:
Synalpheus;bioinformatics;mobile genetic element;genome size;ddRADseq
DOI:
doi:10.5061/dryad.2375033
摘要:
digest restriction-site associated DNA sequencing (ddRADseq) markers contain the same TE compositions as whole genome assemblies across arthropods. Ne
Data from: A RAD-sequencing approach to genome-wide marker discovery, genotyping, and phylogenetic inference in a diverse radiation of primates
负责人:
关键词:
double-digest RAD sequencing;reduced representation sequencing;non-model taxa;phylogenetics;New World primates;SNP markers;20-25 mya;Platyrrhini;restriction enzyme digests
DOI:
doi:10.5061/dryad.85jn3
摘要:
for phylogenetic discordance, including introgression. Our study additionally provides one of the first applications of next-generation sequencing methods
Data from: Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)
负责人:
关键词:
SNP;Reduced Representation Libraries;Coregonus clupeaformis;ddRADseq
DOI:
doi:10.5061/dryad.mf1ck
摘要:
xt-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double
Data from: Adapterama III: quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)
负责人:
关键词:
Sphyrna tiburo;Rhodnius pallescens;Amblyomma americanum;Sphyrna lewini;Sternotherus depressus;Eurycea bislineata;Wisteria;Gambusia affinis;Ixodidae;Kinosternidae
DOI:
doi:10.5061/dryad.tr87dh0
摘要:
robust approach for the construction of dual-digest RADseq libraries that relies on adapters and primers designed in Adapterama I. Major features

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