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Data from: Substantial differences in bias between single-digest and double-digest RAD-seq libraries: a case study
- 负责人:
- DOI:
- doi:10.5061/dryad.qf916
- 摘要:
- The trade?offs of using single?digest vs. double?digest restriction site?associated DNA sequencing (RAD?seq) protocols have been widely discusse
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
- 负责人:
- DOI:
- doi:10.5061/dryad.7tb72
- 摘要:
- e, we developed a protocol and adapters for double-digest RAD sequencing for Ion Torrent (Life Technologies; Ion Proton, Ion PGM) semiconductor sequencing
Data from: Efficiency of ddRAD target enriched sequencing across spiny rock lobster species (Palinuridae: Jasus)
- 负责人:
- DOI:
- doi:10.5061/dryad.3dk40
- 摘要:
- Double digest restriction site-associated DNA sequencing (ddRADseq) and target capture sequencing methods are used to explore population and phyloge
Data from: Phylogenomics of phrynosomatid lizards: conflicting signals from sequence capture versus restriction site associated DNA sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.4vt08
- 摘要:
- are lacking. We compared phylogenies estimated from sequence capture and double digest RADseq (ddRADseq) data for North American phrynosomatid lizards
Data from: Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype calling accuracy
- 负责人:
- DOI:
- doi:10.5061/dryad.34dt1
- 摘要:
- ng it difficult to estimate the extent of PCR duplication. Here, we introduce a slight change to the Illumina sequencing adaptor chemistry, appending a unique
Data from: TERAD: Extraction of transposable element composition from RADseq data
- 负责人:
- DOI:
- doi:10.5061/dryad.2375033
- 摘要:
- digest restriction-site associated DNA sequencing (ddRADseq) markers contain the same TE compositions as whole genome assemblies across arthropods. Ne
Data from: A RAD-sequencing approach to genome-wide marker discovery, genotyping, and phylogenetic inference in a diverse radiation of primates
- 负责人:
- DOI:
- doi:10.5061/dryad.85jn3
- 摘要:
- for phylogenetic discordance, including introgression. Our study additionally provides one of the first applications of next-generation sequencing methods
Data from: Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)
- 负责人:
- DOI:
- doi:10.5061/dryad.mf1ck
- 摘要:
- xt-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double
Data from: Genome-wide assessment of diversity and divergence among extant Galápagos giant tortoise species
- 负责人:
- DOI:
- doi:10.5061/dryad.dm3hb40
- 摘要:
- markers. Taking this next step, we used double-digest restriction-site associated DNA sequencing to collect genotypic data at >26,000
Data from: Adapterama III: quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)
- 负责人:
- DOI:
- doi:10.5061/dryad.tr87dh0
- 摘要:
- robust approach for the construction of dual-digest RADseq libraries that relies on adapters and primers designed in Adapterama I. Major features