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Data from: Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity
- 负责人：
- DOI：
- doi:10.5061/dryad.p3ks4
- 摘要：
- w primer pairs gave PCR improvements up to 30% compared with common-used ones. The new universal ITS primers will find wide application in both plant and fungal
Data from: Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n8v4v6d
- 摘要：
- sequence or ligating adapters onto PCR products. In Adapterama I, we presented universal stubs and primers to produce thousands of unique index combinations
Data from: The choice of universal primers and the characteristics of the species mixture determines when DNA metabarcoding can be quantitative.
- 负责人：
- DOI：
- doi:10.5061/dryad.q2r3b1f
- 摘要：
- targeting the mitochondrial COI region and the mitogenomes of ca. 1200 insect species. The results showed that some primers pairs produced quantitative res
Data from: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical
- 负责人：
- DOI：
- doi:10.5061/dryad.54v2q
- 摘要：
- We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using al
Data from: A broadly applicable COI primer pair and an efficient single?tube amplicon library preparation protocol for metabarcoding
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.5jq5t36
- 摘要：
- , this variability also makes it difficult to design truly universal primers. Here, we present the forward primer “Sauron-S878”, specifically designed to facilitate
Data from: Discriminating plants using the DNA barcode rbcLb: an appraisal based on a large data set
- 负责人：
- DOI：
- doi:10.5061/dryad.qk688
- 摘要：
- h universal and plant group-specific primers were designed to amplify rbcLb, and the performance of rbcLa and rbcLb was tested in several ways. Using BLAST, bot
Data from: Multiplex PCR targeting lineage specific SNPs ? A highly efficient and simple approach to block out predator sequences in molecular
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.44mn451
- 摘要：
- interactions. Metabarcoding from predator gut content extractions with universal primers promises to provide simple and rapid insights into food web inter
Data from: A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application
- 负责人：
- 关键词：
- second generation sequencing DNA barcoding mini-barcode mitochondrial marker trophic interactions food web
- DOI：
- doi:10.5061/dryad.6gd51
- 摘要：
- ) is by far the most widely available sequence region in public reference libraries. This is largely because the available versatile (“universal”) COI primers
Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods
- 负责人：
- DOI：
- doi:10.5061/dryad.2p51n
- 摘要：
- nontarget species to some degree. Here, we accurately estimate the extent of the PCR biases induced by universal and blocking primers on a mock community pr
Data from: Non-specific amplification compromises environmental DNA metabarcoding with COI
- 负责人：
- DOI：
- doi:10.5061/dryad.b8f6s44
- 摘要：
- , cytochrome b, 12S and 16S, in terms of reference library coverage, taxonomic discriminatory power, and primer universality. We subsequently test in vitro thr
