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Data from: Not all sequence tags are created equal: designing and validating sequence identification tags robust to indels
- 负责人：
- DOI：
- doi:10.5061/dryad.4m0v8474
- 摘要：
- Ligating adapters with unique synthetic oligonucleotide sequences (sequence tags) onto individual DNA samples before massively parallel sequencing
Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
- 负责人：
- DOI：
- doi:10.5061/dryad.v7f7r
- 摘要：
- ted whether the estimated frequency of representative sequences, generated by 454 amplicon sequencing, differs significantly from that obtaine
Data from: The phylogenetic utility and functional constraint of microRNA flanking sequences
- 负责人：
- DOI：
- doi:10.5061/dryad.49q24
- 摘要：
- and align these sequences from even fragmentary datasets. We also identify significant sequence conservation in the regions directly flanking miRNA genes
Data from: The impact of paralogy on phylogenomic studies - a case study on annelid relationships
- 负责人：
- DOI：
- doi:10.5061/dryad.4js80
- 摘要：
- Phylogenomic studies based on hundreds of genes derived from expressed sequence tags libraries are increasingly used to reveal the phylogeny of taxa
Data from: Efficient in situ barcode sequencing using padlock probe-based BaristaSeq
- 负责人：
- 关键词：
- in situ sequencing
- DOI：
- doi:10.5061/dryad.5dd92
- 摘要：
- approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices
Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
- 负责人：
- DOI：
- doi:10.5061/dryad.179116t
- 摘要：
- . Molecular analyses of these gene families require high-confidence sequences, but the high sequence similarity of the members can create challenges for sequencing
Data from: Comprehensive experimental fitness landscape and evolutionary network for small RNA
- 负责人：
- DOI：
- doi:10.5061/dryad.3rq8q88
- 摘要：
- . The structure of the evolutionary fitness landscape of RNA would determine natural selection for the first functional sequences. Fitness landscapes ar
Data from: Transposon variants and their effects on gene expression in Arabidopsis
- 负责人：
- DOI：
- doi:10.5061/dryad.8674d
- 摘要：
- nearby gene expression, we investigated genome-wide differences in TEs, siRNAs and gene expression among three Arabidopsis thaliana accessions. Both TE sequence
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人：
- DOI：
- doi:10.5061/dryad.28n8d6t
- 摘要：
- targeting the mRNA sequence extending beyond the dsRNA target region, regardless of the expression levels of the target gene. Our results suggest tha
by next-generation sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.1924
- 摘要：
- ome an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA-Seq. We have conducted a 454 GS-FLX pyrosequenc
