A device for sealing a single incision in a laparoscopic surgery, includes: an operating panel located on the top portion of the device, a sleeve located on the bottom portion of the device, a soft connecting portion two symmetrical handles attached to the external surface of the sleeve a blocking flange attached to the bottom end of the sleeve and two clamping portions disposed on the blocking flange. The sleeve is inserted into the wound of the abdominal wall, the blocking flange is positioned inside the abdominal cavity and the soft connecting portion is positioned outside the abdominal cavity. The operating panel rotates around the axis of the soft connecting portion, and the operating holes with seals may avoid air leakage of the abdominal cavity. The device solves the defect that the use of a tubular anastomat in a laparoscopic surgery easily leads to air leakage and inconvenient operation.