A method of flow cytometry sample processing, comprising the steps of establishing a sheath fluid； injecting a sample into said sheath fluid at an injection point； subjecting said sample to a first axial motion surface in a nozzle； transitioning to a second axial motion surface in said nozzle； subjecting said sample to said second axial motion surface in said nozzle wherein said first and said second axial motion surfaces transition with a maximal acceleration differentiation； coordinating said maximal acceleration differentiation so as to not exceed the practical capabilities of said sample over its length； affirmatively limiting said maximal acceleration differentiation so as to not exceed the practical capabilities of said sample over its length； exiting said sample from said nozzle； analyzing said sample.
